218
5 Qualitative Aspects of Analytical Chemistry
Box 5.7
Examples of commonplace combinations between analytes and reagents of
variable nature used in classical qualitative analysis and identification reactions
Note:The more usual relations are depicted with bold lines.
Combination Examples
Analyte
Reagent
Reaction
1
Fe 3 +
SCW
Formation of a red complex
(SCNFe 2 +)
2
A1 3 +
Morine (L)
Formation of a fluorescent
chelate (AIL 2 )
6
Aldehydes Dinitrophenylhydrazine
Formation of a yellowish
orange product
Analyte
Reagent
Reaction
7
Glucose
1) Glucose oxidase
Formation of H 2 0 2 • which
2) Reagent
oxidizes the reagent to a
coloured product
8
Aflatoxins
1) Solid-phase antibody
ELISA (see Box 5.8)
2) Enzyme-bound antibody
3) Substrate
5 Qualitative Aspects of Analytical Chemistry
Box 5.7
Examples of commonplace combinations between analytes and reagents of
variable nature used in classical qualitative analysis and identification reactions
Note:The more usual relations are depicted with bold lines.
Combination Examples
Analyte
Reagent
Reaction
1
Fe 3 +
SCW
Formation of a red complex
(SCNFe 2 +)
2
A1 3 +
Morine (L)
Formation of a fluorescent
chelate (AIL 2 )
6
Aldehydes Dinitrophenylhydrazine
Formation of a yellowish
orange product
Analyte
Reagent
Reaction
7
Glucose
1) Glucose oxidase
Formation of H 2 0 2 • which
2) Reagent
oxidizes the reagent to a
coloured product
8
Aflatoxins
1) Solid-phase antibody
ELISA (see Box 5.8)
2) Enzyme-bound antibody
3) Substrate