CHAPTER 17 • Characterization of Marine Toxins with LC-ESI-MS
l!l c
OIl
l!!
0
:::I
u::
~
~
·iii
c
. =
~
Qj
a:
so
60
40
20
100
m/zS05
75
50
25
4
m/zS19
0.0
4.0
S.O
TIme (min)
OA
325
a
OA
b
DTX-2
DTX-l
c
12.0
16.0
Fig. 17.2. Analysis of digestive-gland extract of DSP-toxin-contaminated Irish mussels; a by LC with
fluorescence detection of 9-anthryldiazomethane-derivatized toxins; b,c by LC-ESI-MS single ion
chromatograms, obtained from SIM operating mode (reprinted with permission from Pleasance et al.
1992b)
toxins is executed in selected ion monitoring (S1M) by hopping the analyser among
the masses of the various protonated molecular ions, respectively m/z 805 for OA,
DTX-2, DTX-2B, DTX-2C (all positional isomers of OA), m/z 819 for DTX-l (Draisci
l!l c
OIl
l!!
0
:::I
u::
~
~
·iii
c
. =
~
Qj
a:
so
60
40
20
100
m/zS05
75
50
25
4
m/zS19
0.0
4.0
S.O
TIme (min)
OA
325
a
OA
b
DTX-2
DTX-l
c
12.0
16.0
Fig. 17.2. Analysis of digestive-gland extract of DSP-toxin-contaminated Irish mussels; a by LC with
fluorescence detection of 9-anthryldiazomethane-derivatized toxins; b,c by LC-ESI-MS single ion
chromatograms, obtained from SIM operating mode (reprinted with permission from Pleasance et al.
1992b)
toxins is executed in selected ion monitoring (S1M) by hopping the analyser among
the masses of the various protonated molecular ions, respectively m/z 805 for OA,
DTX-2, DTX-2B, DTX-2C (all positional isomers of OA), m/z 819 for DTX-l (Draisci