CHAPTER 10 • Occurence, Pathways and Bioaccumulation of Organometallic Compounds
207
Fig. 10.4. Chromatographic
I
separation of monobutyltin
999
chloride (MBT), dibutyltin
chloride (DBT) and tributyltin
888
chloride (TBT) standard solutions
777
666
555
444
333
222
111
0
I
Seawater sample +
Filtration
throughout 0.45 11m
PC filters 0 0.47 mm
~
Unfiltered
Filtered
800 ml of sea water extracted twice with 80 ml
methylene dichloride / tropolone 0.05% in
separating funnel
I
I
I
I
DBT
I
~
Gills
~
) MBT
Mussel sample
+
Whole
~
tissues
~
Glands
2 g tissues homogenized in
methanol / tropolone 0.05%
+
Extraction with 30 ml methylene dichloride in
separating funnel
I
I Collection of methylene dichloride phase and evaporation to dryness by a vacuum rotary evaporator I
Redissolution in methanol and injection of 200 III aliquots into the HPLC column
Fig. 10.5. Analytical schema for the extraction and preconcentration steps of butyltins from sea water
and mussel tissues
Fig. 10.5. The chromatographic elution and following Hg-ICP/AES determination were
then carried out.