206
Fig. 10.3. Schematic diagram
of HPLC-HG-ICP/AES system
used for organotins determination
R. Frache . P. Rivaro
HPLC column:
Partisil SCX1 OiJ
Injection volume: 200iJi
Eluent:
MeOH 80% - Hp 20%
CH 3 COONH 4 0.1 M
Troplolone 0.1 %
Flow:
1 mlmin-1
....
I HCI 0.3 M
I
....
I Flow: 0.7 ml min- 1
...
I NaBH 4 1% NaOH 1 M
I
....
I Flow: 0.7 ml min- 1
u
I Hydride generation I
~,.
I
I
I
Solvent
Gas-liquid separator ---I~~ washed out
u
Organo Sn hydrides
carried out to
plasma torch
-'''
ICP-AES:
ASn(ll):
189.989 nm
T scansion: 300 ms
The Genoa oil port is on the west side of the town; there are 4 wharves, 250-500 m
long, and it is 11-14 m deep. The terminal handles 500-600 oil tankers each year, which
visit the port for one to two days. Sea water and mussels of a natural population, 5-6 cm
in length, were sampled at two month intervals between July 1997 and May 1998. At
each sampling, 10 I of sea water was collected by a LDPE container, and kept at 4°C
until organotin extraction. At the same time, 25 mussels were collected: the whole soft
tissues of 8 individuals and the digestive glands and the gills of the remainder were
dissected, homogenized and frozen at -20°C until analysis. Organotins extractions
from seawater and mussel tissues and determination procedures are schematized in
Fig. 10.3. Schematic diagram
of HPLC-HG-ICP/AES system
used for organotins determination
R. Frache . P. Rivaro
HPLC column:
Partisil SCX1 OiJ
Injection volume: 200iJi
Eluent:
MeOH 80% - Hp 20%
CH 3 COONH 4 0.1 M
Troplolone 0.1 %
Flow:
1 mlmin-1
....
I HCI 0.3 M
I
....
I Flow: 0.7 ml min- 1
...
I NaBH 4 1% NaOH 1 M
I
....
I Flow: 0.7 ml min- 1
u
I Hydride generation I
~,.
I
I
I
Solvent
Gas-liquid separator ---I~~ washed out
u
Organo Sn hydrides
carried out to
plasma torch
-'''
ICP-AES:
ASn(ll):
189.989 nm
T scansion: 300 ms
The Genoa oil port is on the west side of the town; there are 4 wharves, 250-500 m
long, and it is 11-14 m deep. The terminal handles 500-600 oil tankers each year, which
visit the port for one to two days. Sea water and mussels of a natural population, 5-6 cm
in length, were sampled at two month intervals between July 1997 and May 1998. At
each sampling, 10 I of sea water was collected by a LDPE container, and kept at 4°C
until organotin extraction. At the same time, 25 mussels were collected: the whole soft
tissues of 8 individuals and the digestive glands and the gills of the remainder were
dissected, homogenized and frozen at -20°C until analysis. Organotins extractions
from seawater and mussel tissues and determination procedures are schematized in