Table 9.5 Treatment methods based on the use of various microorganisms for aquaculture waste remediation (parameters, quality control and results)
No. Kind of
waste
Treatment
Parameters
Methodology
Quality control
methods
Results
References
1.
Fisheries
processing
waste
Fermentation
Ash, carbohydrates,
lipids, protein, biomass concentration
Compost extracts
were used as a
substrate for Scytalidium acidophilum
ATCC 26774 (agitation speed of 150
rpm, temperature
of 25.0°C, pH of 2.0
± 0.1, and cultivatoin time 8-10 days)
1. Ash,: combustion in a
muffl e furnace at 600°C
2. Carbohydrates: anthrone
reagent method presented
by Morris (1948)
3. Lipids: method described
by Folch et al. (1957)
4. Protein: multiplying the nitrogen, found by A.O.A.C.
Micro-Kjeldahl Method
47.021 (Anon., 1980) by the
factor 6.25
5. Biomass concentration:
procedure of Martin et al.
(1990)
1. Acid extracts of compost
had higher concentrations
of nutrients (lipids, carbohydrates, and protein) than
compost
2. Substrate for the growth
of acid-tolrant fungus S.
acidophilum
3. Used for the biofi ltration
of liquid and gas effl uents
and in the growth of
mushrooms
Martin, 1999
2.
Fish farm
waste
Integrated marine
cultivation of
Gracilaria chilensis
(Gracilariales,
Rhodophyta)
Carbon, total nitrogen, total phosphorous, agar content
The algae were
placed 10 m (1st
station) and 150 m
west of the cages
(2nd station)and
at 1 km south of
the fi rst farm (3rd
station)
1. Carbon and total nitrogen:
CHN elemental analyser
2. Total phosphorus: extraction using hydrocloric acid
(Aspila et al.,1976)
3. Agar content: according
to Cancino and Orellana
(1987) and expressed at
percentage of Gracihuria
dry weight
1. First station: 40% higher
growth rate of Gracihuria
cultivated compared to the
other stations
2. First station: higher algae
nutrient content close to
the cages (1.9-2.1 mmol N
g
-1 dw
-1 and 0.28-0.34 mmol
Pg
-1 dw
-1
) compared to the
other distances
3. I ha cultivation of the algae,
close to the fi sh cages,
resulted in 5% removel of
dessolved inorganic nitrogen and 27% of released
dissolved phosphorous
Troell et al.,
1997
No. Kind of
waste
Treatment
Parameters
Methodology
Quality control
methods
Results
References
1.
Fisheries
processing
waste
Fermentation
Ash, carbohydrates,
lipids, protein, biomass concentration
Compost extracts
were used as a
substrate for Scytalidium acidophilum
ATCC 26774 (agitation speed of 150
rpm, temperature
of 25.0°C, pH of 2.0
± 0.1, and cultivatoin time 8-10 days)
1. Ash,: combustion in a
muffl e furnace at 600°C
2. Carbohydrates: anthrone
reagent method presented
by Morris (1948)
3. Lipids: method described
by Folch et al. (1957)
4. Protein: multiplying the nitrogen, found by A.O.A.C.
Micro-Kjeldahl Method
47.021 (Anon., 1980) by the
factor 6.25
5. Biomass concentration:
procedure of Martin et al.
(1990)
1. Acid extracts of compost
had higher concentrations
of nutrients (lipids, carbohydrates, and protein) than
compost
2. Substrate for the growth
of acid-tolrant fungus S.
acidophilum
3. Used for the biofi ltration
of liquid and gas effl uents
and in the growth of
mushrooms
Martin, 1999
2.
Fish farm
waste
Integrated marine
cultivation of
Gracilaria chilensis
(Gracilariales,
Rhodophyta)
Carbon, total nitrogen, total phosphorous, agar content
The algae were
placed 10 m (1st
station) and 150 m
west of the cages
(2nd station)and
at 1 km south of
the fi rst farm (3rd
station)
1. Carbon and total nitrogen:
CHN elemental analyser
2. Total phosphorus: extraction using hydrocloric acid
(Aspila et al.,1976)
3. Agar content: according
to Cancino and Orellana
(1987) and expressed at
percentage of Gracihuria
dry weight
1. First station: 40% higher
growth rate of Gracihuria
cultivated compared to the
other stations
2. First station: higher algae
nutrient content close to
the cages (1.9-2.1 mmol N
g
-1 dw
-1 and 0.28-0.34 mmol
Pg
-1 dw
-1
) compared to the
other distances
3. I ha cultivation of the algae,
close to the fi sh cages,
resulted in 5% removel of
dessolved inorganic nitrogen and 27% of released
dissolved phosphorous
Troell et al.,
1997