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1. Pellerito· R. Barbieri· R. Di Stefano . M. Scopelliti . C. Pellerito . T. Fiore· F. Triolo
Table 15.16. Genotoxic activity: mitotic metaphase and anaphase chromosomal damage in
Paracentrotus lividus embryos treated with R3SnCl and R3SnL-HCA (L-HCA = L-homocysteic acid;
R = methyl, butyl and phenyl), adapted from Pellerito et aI. (1997a)
Compound
Concentration Time interval No. of spreads
(moldm- 3 )
(h)
Normal Irregular Granular Colchicinized-like
Total
staining zones
chromosome
spreads
Controls
71
74
6-8
110
4
114
18-20
258
3
264
Me 3 Sn(L-HCA)
10- 5
Arrest
6-8
18-20
10- 7
6
8
8
20
42
6-8
4
6
25
38
18-20
5
5
Me 3 SnCI
10- 5
Arrest
6-8
18-20
10- 7
2
4
10
12
15
41
6-8
4
4
5
20
33
18-20
3
7
10
nBujSn(L-HCA) 10- 5
Arrest
6-8
18-20
10- 7
2
4
6
6
12
28
6-8
0
20
28
18-20
Arrest
nBu 3 SnCl
10- 5
2
Arrest
6-8
18-20
10- 7
7
12
6
15
40
6-8
4
4
26
36
18-20
Arrest
Ph 3 Sn(L -HCA)
10- 5
2
Arrest
6-8
18-20
10- 7
Arrest
6-8
18-20