Improving Marine Algae for Bioenergy 295
Martin, B.A. and P.D. Frymier. 2017. A review of hydrogen production by photosynthetic organisms using whole-cell and
cell-free systems. Appl. Biochem. Biotechnol. 183: 503–519.
Masukawa, H., K. Inoue and H. Sakurai. 2007. Effects of disruption of homocitrate synthase genes on Nostoc sp. strain PCC
7120 photobiological hydrogen production and nitrogenase. Appl. Environ. Microbiol. 73: 7562–7570.
Masukawa, H., K. Inoue, H. Sakurai, C.P. Wolk and R.P. Hausinger. 2010. Site-directed mutagenesis of the Anabaena sp.
strain PCC 7120 nitrogenase active site to increase photobiological hydrogen production. Appl. Environ. Microbiol.
76: 6741–6750.
Masukawa, H., M. Mochimaru and H. Sakurai. 2002. Disruption of the uptake hydrogenase gene, but not of the bidirectional
hydrogenase gene, leads to enhanced photobiological hydrogen production by the nitrogen-fixing cyanobacterium
Anabaena sp. PCC 7120. Appl. Microbiol. Biotechnol. 58: 618–624.
Masukawa, H., M. Kitashima, K. Inoue, H. Sakurai and R.P. Hausinger. 2012. Genetic engineering of cyanobacteria to enhance
biohydrogen production from sunlight and water. Ambio. 41 Suppl 2: 169–173.
Masukawa, H., H. Sakurai, R.P. Hausinger and K. Inoue. 2017. Increased heterocyst frequency by patN disruption in Anabaena
leads to enhanced photobiological hydrogen production at high light intensity and high cell density. Appl. Microbiol.
Biotechnol. 101: 2177–2188.
Mata, T.M., A.A. Martins and N.S. Caetano. 2010. Microalgae for biodiesel production and other applications: a review.
Renew. Sustain. Energy Rev. 14: 217–232.
Matsunaga, T., H. Takeyama and N. Nakamura. 1990. Characterization of cryptic plasmids from marine cyanobacteria and
construction of a hybrid plasmid potentially capable of transformation of marine cyanobacterium, Synechococcus sp.
and its transformation. Appl. Biochem. Biotechnol. 24: 151–160.
Mayer, S.M., C.A. Gormal, B.E. Smith and D.M. Lawson. 2002. Crystallographic analysis of the MoFe protein of nitrogenase
from a nifV mutant of Klebsiella pneumoniae identifies citrate as a ligand to the molybdenum of iron molybdenum
cofactor (FeMoco). J. Biol. Chem. 277: 35263–35266.
McGrath, J.M. and S.P. Long. 2014. Can the cyanobacterial carbon-concentrating mechanism increase photosynthesis in crop
species? A theoretical analysis. Plant Physiol. 164: 2247–2261.
McIntosh, C.L., F. Germer, R. Schulz, J. Appel and A.K. Jones. 2011. The [NiFe]-hydrogenase of the cyanobacterium
Synechocystis sp. PCC 6803 works bidirectionally with a bias to H 2 production. J. Am. Chem. Soc. 133: 11308–11319.
Melis, A., L. Zhang, M. Forestier, M.L. Ghirardi and M. Seibert. 2000. Sustained photobiological hydrogen gas production upon
reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii. Plant Physiol. 122: 127–136.
Merchant, S.S., S.E. Prochnik, O. Vallon, E.H. Harris, S.J. Karpowicz, G.B. Witman, A. Terry, A. Salamov, L.K Fritz-Laylin,
L. Maréchal-Drouard, W.F. Marshall, L.H. Qu, D.R. Nelson, A.A. Sanderfoot, M.H. Spalding, V.V. Kapitonov, Q. Ren,
P. Ferris, E. Lindquist, H. Shapiro, S.M. Lucas, J. Grimwood, J. Schmutz, P. Cardol, H. Cerutti, G. Chanfreau, C.L.
Chen, V. Cognat, M.T. Croft, R. Dent, S. Dutcher, E. Fernández, H. Fukuzawa, D. González-Ballester, D. GonzálezHalphen, A. Hallmann, M. Hanikenne, M. Hippler, W. Inwood, K. Jabbari, M. Kalanon, R. Kuras, P.A. Lefebvre, S.D.
Lemaire, A.V. Lobanov, M. Lohr, A. Manuell, I. Meier, L. Mets, M. Mittag, T. Mittelmeier, J.V. Moroney, J. Moseley,
C. Napoli, A.M. Nedelcu, K. Niyogi, S.V. Novoselov, I.T. Paulsen, G. Pazour, S. Purton, J.P. Ral, D.M. Riaño-Pachón,
W. Riekhof, L. Rymarquis, M. Schroda, D. Stern, J. Umen, R. Willows, N. Wilson, S.L. Zimmer, J. Allmer, J. Balk, K.
Bisova, C.J. Chen, M. Elias, K. Gendler, C. Hauser, M.R. Lamb, H. Ledford, J.C. Long, J. Minagawa, M.D. Page, J.
Pan, W. Pootakham, S. Roje, A. Rose, E. Stahlberg, A.M. Terauchi, P. Yang, S. Ball, C. Bowler, C.L. Dieckmann, V.N.
Gladyshev, P. Green, R. Jorgensen, S. Mayfield, B. Mueller-Roeber, S. Rajamani, R.T. Sayre, P. Brokstein, I. Dubchak,
D. Goodstein, L. Hornick, Y.W. Huang, J. Jhaveri, Y. Luo, D. Martínez, W.C. Ngau, B. Otillar, A. Poliakov, A. Porter, L.
Szajkowski, G. Werner, K. Zhou, I.V. Grigoriev, D.S. Rokhsar and A.R. Grossman. 2007. The Chlamydomonas genome
reveals the evolution of key animal and plant functions. Science 318: 245–250.
Meuser, J.E., G. Ananyev, L.E. Wittig, S. Kosourov, M.L. Ghirardi, M. Seibert, G.C. Dismukes and M.C. Posewitz. 2009.
Phenotypic diversity of hydrogen production in chlorophycean algae reflects distinct anaerobic metabolisms. J. Biotechnol.
142: 21–30.
Meuser, J.E., S. D’Adamo, R.E. Jinkerson, F. Mus, W. Yang, M.L. Ghirardi, M. Seibert, A.R. Grossman and M.C. Posewitz.
2012. Genetic disruption of both Chlamydomonas reinhardtii [FeFe]-hydrogenases: Insight into the role of HYDA2 in
H 2 production. Biochem. Biophys. Res. Commun. 417: 704–709.
Miyagawa, Y., M. Tamoi and S. Shigeoka. 2001. Overexpression of a cyanobacterial fructose-1,6-/sedoheptulose-1,7bisphosphatase in tobacco enhances photosynthesis and growth. Nat. Biotechnol. 19: 965–969.
Mielke, S.P., N.Y. Kiang, R.E. Blankenship, M.R. Gunner and D. Mauzerall. 2011. Efficiency of photosynthesis in a Chl
d-utilizing cyanobacterium is comparable to or higher than in Chl a-utilizing oxygenic species. Biochim. Biophys. Acta
1807: 1231–1236.
Mueller-Cajar, O. and S.M. Whitney. 2008a. Evolving improved Synechococcus Rubisco functional expression in Escherichia
coli. Biochem. J. 414: 205–214.
Mueller-Cajar, O. and S.M. Whitney. 2008b. Directing the evolution of Rubisco and Rubisco activase: first impressions of a
new tool for photosynthesis research. Photosynth. Res. 98: 667–675.
Mulder, D.W., E.S. Boyd, R. Sarma, R.K. Lange, J.A. Endrizzi, J.B. Broderick and J.W. Peters. 2010. Stepwise [FeFe]hydrogenase H-cluster assembly revealed in the structure of HydA(DeltaEFG). Nature 465: 248–251.
Martin, B.A. and P.D. Frymier. 2017. A review of hydrogen production by photosynthetic organisms using whole-cell and
cell-free systems. Appl. Biochem. Biotechnol. 183: 503–519.
Masukawa, H., K. Inoue and H. Sakurai. 2007. Effects of disruption of homocitrate synthase genes on Nostoc sp. strain PCC
7120 photobiological hydrogen production and nitrogenase. Appl. Environ. Microbiol. 73: 7562–7570.
Masukawa, H., K. Inoue, H. Sakurai, C.P. Wolk and R.P. Hausinger. 2010. Site-directed mutagenesis of the Anabaena sp.
strain PCC 7120 nitrogenase active site to increase photobiological hydrogen production. Appl. Environ. Microbiol.
76: 6741–6750.
Masukawa, H., M. Mochimaru and H. Sakurai. 2002. Disruption of the uptake hydrogenase gene, but not of the bidirectional
hydrogenase gene, leads to enhanced photobiological hydrogen production by the nitrogen-fixing cyanobacterium
Anabaena sp. PCC 7120. Appl. Microbiol. Biotechnol. 58: 618–624.
Masukawa, H., M. Kitashima, K. Inoue, H. Sakurai and R.P. Hausinger. 2012. Genetic engineering of cyanobacteria to enhance
biohydrogen production from sunlight and water. Ambio. 41 Suppl 2: 169–173.
Masukawa, H., H. Sakurai, R.P. Hausinger and K. Inoue. 2017. Increased heterocyst frequency by patN disruption in Anabaena
leads to enhanced photobiological hydrogen production at high light intensity and high cell density. Appl. Microbiol.
Biotechnol. 101: 2177–2188.
Mata, T.M., A.A. Martins and N.S. Caetano. 2010. Microalgae for biodiesel production and other applications: a review.
Renew. Sustain. Energy Rev. 14: 217–232.
Matsunaga, T., H. Takeyama and N. Nakamura. 1990. Characterization of cryptic plasmids from marine cyanobacteria and
construction of a hybrid plasmid potentially capable of transformation of marine cyanobacterium, Synechococcus sp.
and its transformation. Appl. Biochem. Biotechnol. 24: 151–160.
Mayer, S.M., C.A. Gormal, B.E. Smith and D.M. Lawson. 2002. Crystallographic analysis of the MoFe protein of nitrogenase
from a nifV mutant of Klebsiella pneumoniae identifies citrate as a ligand to the molybdenum of iron molybdenum
cofactor (FeMoco). J. Biol. Chem. 277: 35263–35266.
McGrath, J.M. and S.P. Long. 2014. Can the cyanobacterial carbon-concentrating mechanism increase photosynthesis in crop
species? A theoretical analysis. Plant Physiol. 164: 2247–2261.
McIntosh, C.L., F. Germer, R. Schulz, J. Appel and A.K. Jones. 2011. The [NiFe]-hydrogenase of the cyanobacterium
Synechocystis sp. PCC 6803 works bidirectionally with a bias to H 2 production. J. Am. Chem. Soc. 133: 11308–11319.
Melis, A., L. Zhang, M. Forestier, M.L. Ghirardi and M. Seibert. 2000. Sustained photobiological hydrogen gas production upon
reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii. Plant Physiol. 122: 127–136.
Merchant, S.S., S.E. Prochnik, O. Vallon, E.H. Harris, S.J. Karpowicz, G.B. Witman, A. Terry, A. Salamov, L.K Fritz-Laylin,
L. Maréchal-Drouard, W.F. Marshall, L.H. Qu, D.R. Nelson, A.A. Sanderfoot, M.H. Spalding, V.V. Kapitonov, Q. Ren,
P. Ferris, E. Lindquist, H. Shapiro, S.M. Lucas, J. Grimwood, J. Schmutz, P. Cardol, H. Cerutti, G. Chanfreau, C.L.
Chen, V. Cognat, M.T. Croft, R. Dent, S. Dutcher, E. Fernández, H. Fukuzawa, D. González-Ballester, D. GonzálezHalphen, A. Hallmann, M. Hanikenne, M. Hippler, W. Inwood, K. Jabbari, M. Kalanon, R. Kuras, P.A. Lefebvre, S.D.
Lemaire, A.V. Lobanov, M. Lohr, A. Manuell, I. Meier, L. Mets, M. Mittag, T. Mittelmeier, J.V. Moroney, J. Moseley,
C. Napoli, A.M. Nedelcu, K. Niyogi, S.V. Novoselov, I.T. Paulsen, G. Pazour, S. Purton, J.P. Ral, D.M. Riaño-Pachón,
W. Riekhof, L. Rymarquis, M. Schroda, D. Stern, J. Umen, R. Willows, N. Wilson, S.L. Zimmer, J. Allmer, J. Balk, K.
Bisova, C.J. Chen, M. Elias, K. Gendler, C. Hauser, M.R. Lamb, H. Ledford, J.C. Long, J. Minagawa, M.D. Page, J.
Pan, W. Pootakham, S. Roje, A. Rose, E. Stahlberg, A.M. Terauchi, P. Yang, S. Ball, C. Bowler, C.L. Dieckmann, V.N.
Gladyshev, P. Green, R. Jorgensen, S. Mayfield, B. Mueller-Roeber, S. Rajamani, R.T. Sayre, P. Brokstein, I. Dubchak,
D. Goodstein, L. Hornick, Y.W. Huang, J. Jhaveri, Y. Luo, D. Martínez, W.C. Ngau, B. Otillar, A. Poliakov, A. Porter, L.
Szajkowski, G. Werner, K. Zhou, I.V. Grigoriev, D.S. Rokhsar and A.R. Grossman. 2007. The Chlamydomonas genome
reveals the evolution of key animal and plant functions. Science 318: 245–250.
Meuser, J.E., G. Ananyev, L.E. Wittig, S. Kosourov, M.L. Ghirardi, M. Seibert, G.C. Dismukes and M.C. Posewitz. 2009.
Phenotypic diversity of hydrogen production in chlorophycean algae reflects distinct anaerobic metabolisms. J. Biotechnol.
142: 21–30.
Meuser, J.E., S. D’Adamo, R.E. Jinkerson, F. Mus, W. Yang, M.L. Ghirardi, M. Seibert, A.R. Grossman and M.C. Posewitz.
2012. Genetic disruption of both Chlamydomonas reinhardtii [FeFe]-hydrogenases: Insight into the role of HYDA2 in
H 2 production. Biochem. Biophys. Res. Commun. 417: 704–709.
Miyagawa, Y., M. Tamoi and S. Shigeoka. 2001. Overexpression of a cyanobacterial fructose-1,6-/sedoheptulose-1,7bisphosphatase in tobacco enhances photosynthesis and growth. Nat. Biotechnol. 19: 965–969.
Mielke, S.P., N.Y. Kiang, R.E. Blankenship, M.R. Gunner and D. Mauzerall. 2011. Efficiency of photosynthesis in a Chl
d-utilizing cyanobacterium is comparable to or higher than in Chl a-utilizing oxygenic species. Biochim. Biophys. Acta
1807: 1231–1236.
Mueller-Cajar, O. and S.M. Whitney. 2008a. Evolving improved Synechococcus Rubisco functional expression in Escherichia
coli. Biochem. J. 414: 205–214.
Mueller-Cajar, O. and S.M. Whitney. 2008b. Directing the evolution of Rubisco and Rubisco activase: first impressions of a
new tool for photosynthesis research. Photosynth. Res. 98: 667–675.
Mulder, D.W., E.S. Boyd, R. Sarma, R.K. Lange, J.A. Endrizzi, J.B. Broderick and J.W. Peters. 2010. Stepwise [FeFe]hydrogenase H-cluster assembly revealed in the structure of HydA(DeltaEFG). Nature 465: 248–251.