Improving Marine Algae for Bioenergy 293
Durao, P., H. Aigner, P. Nagy, O. Mueller-Cajar, F.U. Hayer-Hartl and M. Hartl. 2015. Opposing effects of folding and assembly
chaperones on evolvability of Rubisco. Nat. Chem. Biol. 11: 148–155.
Eilenberg, H., I. Weiner, O. Ben-Zvi, C. Pundak, A. Marmari, O. Liran, M.S. Wecker, Y. Milrad and I. Yacoby. 2016. The
dual effect of a ferredoxin-hydrogenase fusion protein in vivo: successful divergence of the photosynthetic electron flux
towards hydrogen production and elevated oxygen tolerance. Biotechnol. Biofuels 9: 182.
Eroglu, E. and A. Melis. 2011. Photobiological hydrogen production: Recent advances and state of the art. Bioresour. Technol.
102: 8403–8413.
Esquível, M.G., T.S. Pinto, J. Marín-Navarro and J. Moreno. 2006. Substitution of tyrosine residues at the aromatic cluster
around the betaA-betaB loop of rubisco small subunit affects the structural stability of the enzyme and the in vivo
degradation under stress conditions. Biochemistry 45: 5745–5753.
Esquível, M.G., H.M. Amaro, T.S. Pinto, P.S. Fevereiro and F.X. Malcata. 2011. Efficient H 2 production via Chlamydomonas
reinhardtii. Trends Biotechnol. 29: 595–600.
Esquível, M.G., A.R. Matos and J. Marques Silva. 2017. Rubisco mutants of Chlamydomonas reinhardtii display divergente
photosynthetic parameters and lipid allocation. Appl. Microbiol. Biotechnol. 101: 5569–5580.
Feng, S.Y., L.X. Xue, H.T. Liu and P.J. Lu. 2009. Improvement of efficiency of genetic transformation for Dunaliella salina
by glass beads method. Mol. Biol. Rep. 36: 1433–1439.
Gan, S.Y., S. Qin, R.Y. Othman, D. Yu and S.M. Phang. 2003. Transient expression of lacZ in particle bombarded Gracilaria
changii (Gracilariales, Rhodophyta). J. Appl. Phycol. 15: 345–349.
Ghirardi, M.L., M.C. Posewitz, P.C. Maness, A. Dubini, J. Yu and M. Seibert. 2007. Hydrogenases and hydrogen photoproduction
in oxygenic photosynthetic organisms. Annu Rev. Plant Biol. 58: 71–91.
Ghirardi, M.L., R.K. Togasaki and M. Seibert. 1997. Oxygen sensitivity of algal H 2 -production. Appl. Biochem. Biotechnol.
63: 141–151.
Ghirardi, M.L., P.W. King, M.C. Posewitz, P.C. Maness, A. Fedorov, K. Kim, J. Cohen, K. Schulten and M. Seibert. 2005.
Approaches to developing biological H 2 -photoproducing organisms and processes. Biochem. Soc. Trans. 33: 70–72.
Ghirardi, M.L. 2015. Implementation of photobiological H 2 production: the O 2 sensitivity of hydrogenases. Photosynth. Res.
125: 383–393.
González-Ballester, D., J.L. Jurado-Oller and E. Fernandez. 2015. Relevance of nutrient media composition for hydrogen
production in Chlamydomonas. Photosynth. Res. 125: 395–406.
Gomaa, M.A., L. Al-Haj and R.M.M. Abed. 2016. Metabolic engineering of cyanobacteria and microalgae for enhanced
production of biofuels and high-value products. J. Appl. Microbiol. 121: 919–931.
Goodenough, U., I. Blaby, D. Casero, S.D. Gallaher, C. Goodson, S. Johnson J.H. Lee, S.S. Merchant, M. Pellegrini, R. Roth,
J. Rusch, M. Singh, J.G. Umen, T.L. Weiss and T. Wulan. 2014. The path to triacylglyceride obesity in the sta6 strain
of Chlamydomonas reinhardtii. Eukaryot Cell. 13: 591–613.
Greene, D.N., S.M. Whitney and I. Matsumura. 2007. Artificially evolved Synechococcus PCC6301 Rubisco variants exhibit
improvements in folding and catalytic efficiency. Biochem. J. 404: 517–524.
Gressel, J., D. Eisenstadt, D. Schatz, S. Einbinder and S. Ufaz. 2010. Use of fluorescent protein in cyanobacteria and algae
for improving photosynthesis and preventing cell damage. US Patent US20100087006.
Haimovich-Dayan, M., N. Garfinkel and D. Ewe. 2013. The role of C4 metabolism in the marine diatom Phaeodactylum
tricornutum. New Phytol. 197: 177–185.
Hanson, M.R., M.T. Lin, A.E. Carmo-Silva and M.A.J. Parry. 2016. Towards engineering carboxysomes in C 3 plants. Plant
J. 87: 38–50.
Happe, T., K. Schutz and H. Bohme. 2000. Transcriptional and mutational analysis of the uptake hydrogenase of the filamentous
cyanobacterium Anabaena variabilis ATCC 29413. J. Bacteriol. 182: 1624–1631.
Harris, E.H., D.B. Stern and G.B. Witman. 2009. The Chlamydomonas Sourcebook. Elsevier Science and Technology, New
York, 2nd Ed.
Hawkins, R.L. and M. Nakamura. 1999. Expression of human growth hormone by the eukaryotic alga, Chlorella. Curr.
Microbiol. 38: 335–341.
Hedge, K., N. Chandra, S.J. Sarma, S.K. Brar and V.D. Veeranki. 2015. Genetic engineering strategies for enhanced biodiesel
production. Mol. Biotechnol. 57: 606–624.
Hellingwerf, K.J. and M.J. Teixeira de Mattos. 2009. Alternative routes to biofuels: Light-driven biofuel formation from CO 2
and water based on the “photanol” approach. J. Biotechnol. 142: 87–90.
Hemschemeier, A., S. Fouchard, L. Cournac, G. Peltier and T. Happe. 2008. Hydrogen production by Chlamydomonas
reinhardtii: an elaborate interplay of electron sources and sinks. Planta 227: 397–407.
Hirata, R., M. Takahashi, N. Saga and K. Mikami. 2011. Transient gene expression system established in Porphyra yezoensis
is widely applicable in Bangiophycean algae. Mar. Biotechnol. 13: 1038–1047.
Ho, S.H., X. Ye, T. Hasunuma, J.S. Chang and A. Kondo. 2014. Perspectives on engineering strategies for improving biofuel
production from microalgae—A critical review. Biotechnol. Adv. 32: 1448–1459.
Huang, X., J.C. Weber, T.K. Hinson, A.C. Mathieson and S.C. Minocha. 1996. Transient expression of the GUS reporter gene
in the protoplasts and partially digested cells of Ulva lactuca L. (Chlorophyta). Bot. Mar. 39: 467–474.
Ihara, M., H. Nishihara, K.S. Yoon, O. Lenz, B. Friedrich, H. Nakamoto, K. Kojima, D. Honma, T. Kamachi and I. Okura. 2006.
Light-driven hydrogen production by a hybrid complex of a [NiFe]-hydrogenase and the cyanobacterial photosystem
I. Photochem. Photobiol. 82: 676–682.
Durao, P., H. Aigner, P. Nagy, O. Mueller-Cajar, F.U. Hayer-Hartl and M. Hartl. 2015. Opposing effects of folding and assembly
chaperones on evolvability of Rubisco. Nat. Chem. Biol. 11: 148–155.
Eilenberg, H., I. Weiner, O. Ben-Zvi, C. Pundak, A. Marmari, O. Liran, M.S. Wecker, Y. Milrad and I. Yacoby. 2016. The
dual effect of a ferredoxin-hydrogenase fusion protein in vivo: successful divergence of the photosynthetic electron flux
towards hydrogen production and elevated oxygen tolerance. Biotechnol. Biofuels 9: 182.
Eroglu, E. and A. Melis. 2011. Photobiological hydrogen production: Recent advances and state of the art. Bioresour. Technol.
102: 8403–8413.
Esquível, M.G., T.S. Pinto, J. Marín-Navarro and J. Moreno. 2006. Substitution of tyrosine residues at the aromatic cluster
around the betaA-betaB loop of rubisco small subunit affects the structural stability of the enzyme and the in vivo
degradation under stress conditions. Biochemistry 45: 5745–5753.
Esquível, M.G., H.M. Amaro, T.S. Pinto, P.S. Fevereiro and F.X. Malcata. 2011. Efficient H 2 production via Chlamydomonas
reinhardtii. Trends Biotechnol. 29: 595–600.
Esquível, M.G., A.R. Matos and J. Marques Silva. 2017. Rubisco mutants of Chlamydomonas reinhardtii display divergente
photosynthetic parameters and lipid allocation. Appl. Microbiol. Biotechnol. 101: 5569–5580.
Feng, S.Y., L.X. Xue, H.T. Liu and P.J. Lu. 2009. Improvement of efficiency of genetic transformation for Dunaliella salina
by glass beads method. Mol. Biol. Rep. 36: 1433–1439.
Gan, S.Y., S. Qin, R.Y. Othman, D. Yu and S.M. Phang. 2003. Transient expression of lacZ in particle bombarded Gracilaria
changii (Gracilariales, Rhodophyta). J. Appl. Phycol. 15: 345–349.
Ghirardi, M.L., M.C. Posewitz, P.C. Maness, A. Dubini, J. Yu and M. Seibert. 2007. Hydrogenases and hydrogen photoproduction
in oxygenic photosynthetic organisms. Annu Rev. Plant Biol. 58: 71–91.
Ghirardi, M.L., R.K. Togasaki and M. Seibert. 1997. Oxygen sensitivity of algal H 2 -production. Appl. Biochem. Biotechnol.
63: 141–151.
Ghirardi, M.L., P.W. King, M.C. Posewitz, P.C. Maness, A. Fedorov, K. Kim, J. Cohen, K. Schulten and M. Seibert. 2005.
Approaches to developing biological H 2 -photoproducing organisms and processes. Biochem. Soc. Trans. 33: 70–72.
Ghirardi, M.L. 2015. Implementation of photobiological H 2 production: the O 2 sensitivity of hydrogenases. Photosynth. Res.
125: 383–393.
González-Ballester, D., J.L. Jurado-Oller and E. Fernandez. 2015. Relevance of nutrient media composition for hydrogen
production in Chlamydomonas. Photosynth. Res. 125: 395–406.
Gomaa, M.A., L. Al-Haj and R.M.M. Abed. 2016. Metabolic engineering of cyanobacteria and microalgae for enhanced
production of biofuels and high-value products. J. Appl. Microbiol. 121: 919–931.
Goodenough, U., I. Blaby, D. Casero, S.D. Gallaher, C. Goodson, S. Johnson J.H. Lee, S.S. Merchant, M. Pellegrini, R. Roth,
J. Rusch, M. Singh, J.G. Umen, T.L. Weiss and T. Wulan. 2014. The path to triacylglyceride obesity in the sta6 strain
of Chlamydomonas reinhardtii. Eukaryot Cell. 13: 591–613.
Greene, D.N., S.M. Whitney and I. Matsumura. 2007. Artificially evolved Synechococcus PCC6301 Rubisco variants exhibit
improvements in folding and catalytic efficiency. Biochem. J. 404: 517–524.
Gressel, J., D. Eisenstadt, D. Schatz, S. Einbinder and S. Ufaz. 2010. Use of fluorescent protein in cyanobacteria and algae
for improving photosynthesis and preventing cell damage. US Patent US20100087006.
Haimovich-Dayan, M., N. Garfinkel and D. Ewe. 2013. The role of C4 metabolism in the marine diatom Phaeodactylum
tricornutum. New Phytol. 197: 177–185.
Hanson, M.R., M.T. Lin, A.E. Carmo-Silva and M.A.J. Parry. 2016. Towards engineering carboxysomes in C 3 plants. Plant
J. 87: 38–50.
Happe, T., K. Schutz and H. Bohme. 2000. Transcriptional and mutational analysis of the uptake hydrogenase of the filamentous
cyanobacterium Anabaena variabilis ATCC 29413. J. Bacteriol. 182: 1624–1631.
Harris, E.H., D.B. Stern and G.B. Witman. 2009. The Chlamydomonas Sourcebook. Elsevier Science and Technology, New
York, 2nd Ed.
Hawkins, R.L. and M. Nakamura. 1999. Expression of human growth hormone by the eukaryotic alga, Chlorella. Curr.
Microbiol. 38: 335–341.
Hedge, K., N. Chandra, S.J. Sarma, S.K. Brar and V.D. Veeranki. 2015. Genetic engineering strategies for enhanced biodiesel
production. Mol. Biotechnol. 57: 606–624.
Hellingwerf, K.J. and M.J. Teixeira de Mattos. 2009. Alternative routes to biofuels: Light-driven biofuel formation from CO 2
and water based on the “photanol” approach. J. Biotechnol. 142: 87–90.
Hemschemeier, A., S. Fouchard, L. Cournac, G. Peltier and T. Happe. 2008. Hydrogen production by Chlamydomonas
reinhardtii: an elaborate interplay of electron sources and sinks. Planta 227: 397–407.
Hirata, R., M. Takahashi, N. Saga and K. Mikami. 2011. Transient gene expression system established in Porphyra yezoensis
is widely applicable in Bangiophycean algae. Mar. Biotechnol. 13: 1038–1047.
Ho, S.H., X. Ye, T. Hasunuma, J.S. Chang and A. Kondo. 2014. Perspectives on engineering strategies for improving biofuel
production from microalgae—A critical review. Biotechnol. Adv. 32: 1448–1459.
Huang, X., J.C. Weber, T.K. Hinson, A.C. Mathieson and S.C. Minocha. 1996. Transient expression of the GUS reporter gene
in the protoplasts and partially digested cells of Ulva lactuca L. (Chlorophyta). Bot. Mar. 39: 467–474.
Ihara, M., H. Nishihara, K.S. Yoon, O. Lenz, B. Friedrich, H. Nakamoto, K. Kojima, D. Honma, T. Kamachi and I. Okura. 2006.
Light-driven hydrogen production by a hybrid complex of a [NiFe]-hydrogenase and the cyanobacterial photosystem
I. Photochem. Photobiol. 82: 676–682.