90
S.L. Chang
suspensions were added to the water to give each a calculated density of 5,000 ml"
1
. The
bacteria-laden water was dispensed in 40-ml amounts in 3 petri dishes, and each dish
received enough suspensions of both strains of Naegleria to give a calculated amoeba
density of 50 ml"
1 of each strain.
At weekly intervals of incubation at room temperature 3-ml samples were taken and
processed for plate plaquing for enumerating the wild strain of TV. gruberi and another set
4x10·
5
1x10
J
1x10
1x10
1x10'
1x10
O
Wild s t r a i n
Φ . . . . Pathogenic s t r a i n
TIME (week)
Fig. 1
Growth of wild and pathogenic strains of N. gruben in simulated natural aquatic environment at 25°C.
S.L. Chang
suspensions were added to the water to give each a calculated density of 5,000 ml"
1
. The
bacteria-laden water was dispensed in 40-ml amounts in 3 petri dishes, and each dish
received enough suspensions of both strains of Naegleria to give a calculated amoeba
density of 50 ml"
1 of each strain.
At weekly intervals of incubation at room temperature 3-ml samples were taken and
processed for plate plaquing for enumerating the wild strain of TV. gruberi and another set
4x10·
5
1x10
J
1x10
1x10
1x10'
1x10
O
Wild s t r a i n
Φ . . . . Pathogenic s t r a i n
TIME (week)
Fig. 1
Growth of wild and pathogenic strains of N. gruben in simulated natural aquatic environment at 25°C.