Pathogenic Free-Living Amoebae and Recreational Waters
Table 2. Pathogenicity of Wild and Pathogenic Strains of Naegleria
95
Strains and Origin
CPE on Mammalian Cells
Pathogenicity on mice*
Wild strains o/N. gruberi
2 strains from sewage
effluents
No CPE on PMK and Vera
cells; some CPE on Hep-2
cells
Nonpathogenic to mice by
either intranasal or
intracerebral inoculation
1 strain from Ky lake and
2 from Ohio and Missouri
Rivers
No CPE on PMK and Vera
cells; some CPE on Hep-2
cells
Nonpathogenic to mice by
either intranasal or
intracerebral inoculation
3 swimming pool strains
No CPE on PMK and Vera
cells; some CPE on Hep-2
cells
Nonpathogenic to mice by
either intranasal or
intracerebral inoculation
Pathogenic strains of Naegleria
2 strains from Florida
Marked CPE on MK cells;
Causing fatal meningono growth in Vera or Hep-2 encephalitis in mice by either
cell cultures
intranasal or intracerebral
inoculation
1 strain from Czechoslovakia
2 strains from Australia
3 strains from Richmond,
Virginia
*Most of these results were obtained in cooperative studies with Dr. C. G. Culbertson of Lilly
Research Laboratories and Dr. Richard J. Duma of Virginia Medical Center. These results will be
presented in detail in another report.
REFERENCES
APPLEY, J., CLARKE, S.K.R., ROOME, A.P.C.H., SANDRY, S.A., SAYGI, G., SILK, B., and
WARHURST, D.C. (1970). Primary amoebic meningoencephalitis in Britain. Brit. Med. J., 1,
596-599.
BERG, G., HARRIS, E.K., CHANG, S.L., and BUSCH, K.A. (1963). Quantitation of viruses by the
plaque technique. J. Bacteriol. 85, 691-700.
CARTER, R.E. (1968). Primary amoebic meningo-encephalitis: Clinical, Pathological, and
epidemiological features of six fatal cases. J. Pathol. Bacteriol. 96, 1-25.
CERVA, L. (1971). Studies of limax amoebae in a swimming pool. Hydrobiol. 38, 141-161.
CHANG, S.L. (1958). Cultural, cytological, and ecological observations on the amoeba stage of
Naegleria gruberi J. Gen. Microbiol. 18, 565-578.
CHANG, S.L. (1960). Growth of small free-living amoebae in various bacterial and in bacteria-free
cultures. Can. J. Microbiol. 6, 397-405.
Table 2. Pathogenicity of Wild and Pathogenic Strains of Naegleria
95
Strains and Origin
CPE on Mammalian Cells
Pathogenicity on mice*
Wild strains o/N. gruberi
2 strains from sewage
effluents
No CPE on PMK and Vera
cells; some CPE on Hep-2
cells
Nonpathogenic to mice by
either intranasal or
intracerebral inoculation
1 strain from Ky lake and
2 from Ohio and Missouri
Rivers
No CPE on PMK and Vera
cells; some CPE on Hep-2
cells
Nonpathogenic to mice by
either intranasal or
intracerebral inoculation
3 swimming pool strains
No CPE on PMK and Vera
cells; some CPE on Hep-2
cells
Nonpathogenic to mice by
either intranasal or
intracerebral inoculation
Pathogenic strains of Naegleria
2 strains from Florida
Marked CPE on MK cells;
Causing fatal meningono growth in Vera or Hep-2 encephalitis in mice by either
cell cultures
intranasal or intracerebral
inoculation
1 strain from Czechoslovakia
2 strains from Australia
3 strains from Richmond,
Virginia
*Most of these results were obtained in cooperative studies with Dr. C. G. Culbertson of Lilly
Research Laboratories and Dr. Richard J. Duma of Virginia Medical Center. These results will be
presented in detail in another report.
REFERENCES
APPLEY, J., CLARKE, S.K.R., ROOME, A.P.C.H., SANDRY, S.A., SAYGI, G., SILK, B., and
WARHURST, D.C. (1970). Primary amoebic meningoencephalitis in Britain. Brit. Med. J., 1,
596-599.
BERG, G., HARRIS, E.K., CHANG, S.L., and BUSCH, K.A. (1963). Quantitation of viruses by the
plaque technique. J. Bacteriol. 85, 691-700.
CARTER, R.E. (1968). Primary amoebic meningo-encephalitis: Clinical, Pathological, and
epidemiological features of six fatal cases. J. Pathol. Bacteriol. 96, 1-25.
CERVA, L. (1971). Studies of limax amoebae in a swimming pool. Hydrobiol. 38, 141-161.
CHANG, S.L. (1958). Cultural, cytological, and ecological observations on the amoeba stage of
Naegleria gruberi J. Gen. Microbiol. 18, 565-578.
CHANG, S.L. (1960). Growth of small free-living amoebae in various bacterial and in bacteria-free
cultures. Can. J. Microbiol. 6, 397-405.