l
‘
l.
Immune system of Cyprinid fish
439
cytotoxicity, mixed leucocyte reaction (MLR), macrophage migration in—
(MI) and antigen induced blastogenesis of lymphocytes.
‘
”
The kinetics of cellular immune reactions in cyprinid fish haVe been
ex—
"
studied by transplantations of scales and skin in goldfish and carp
The processes
involved in rejection have been reviewed by’ Hil—
’
(1970),
and
Botham et al.
(1980).
Although lymphocytes and mononu—
phagocytic cells are involved in rejection,- the
exact
meChanisms
not
well
understood e.g.
up
to
now
no
specific cytotoxic lymphocytes
”could be demonstrated.
At optimal temperatures allografts
were
always
rejected
in‘ an
acute
«
”manner,
the
medial
survival
time
(MST) less than 14 days (Rijkers, l982b).
example:
In goldfish et 30°C the
MST
of
first—
and
second—set
scale
were
7.2 and 4.7 days respectively.
No
significant antigen dosé effect
the MST of both first— and second—set allografts was
monitored.
‘
Moreover
‘secondary immune responses were already obtained 7 days after the first graft—
(Hildemann, 1970).
Rijkers (1982b) concluded that in the allograft rejec—
of
teleosts
most
immunological characteristics are comparable to those
'fbund in mammals, with
the
most
important
feature:_
the
formation
of
a
donor specific immunological memory.
‘
The in vitro test for histo—incompatibility, the MLR has
beendescribed
f°r“ carp (—Ca”spi—â Avtalion, 1984;
Gloudemàns et al-, 1985) -
It” Prdvéd *an“afPt
“method for MHC typing of carp (Gloudemans et al., 1985) and for
Studying- the
effect
(Grondel et al., 1985):‘ Grondel &
showed that in a MLR of carp pronephric leucocytes,
”lymphodyte
’growth
promoting
factors
releaSed into
the culture mediùm, re9èmbling
interleukin 2 (IL—2):
'
‘-"
No clear data are available on DTH réadtions in teleost
its
vitr0"manifestationg
test, in cyprinid
“The
ÎËÀ
“gâVe ”positive
g
‘et’
alé,
19825)'it
Was
shown that
fa6t0rs were inVQIVed
in
“this reaction;
for the supernatant of à M1 reaction by
con A, frdm Which
been'removed; Still had inhibitory
Antigen induced
lymph0cytes
been
in”
were?
bath against the pathogen
Although none of the fish developed
antibody,
natural
et
“Èàmers & Ideler
studied
from head kidney of vàCCînated
”ÎäÈëäïEÿ—îhé bacterial preparation,
fish were stimulated as “wgil,
faltoügh
that it Was a real manifestation
animals
to
A.
hydrophila
‘
of i1mùô
.
..
Jï‘t‘hat
E'?“Ë?ÈFÏÇWlËh; t_hÎ
añ—*
cas eof wpo’s
…,« ”indicates that
*ièrôïÿï‘f(°f tln>hbndV1pd
AS a1ready mentioned
“" I 980 ;
»' Ri“ jke r's‘ é tal
,
19 8056
,
\î Ï
,
_
Â
…
\\\
\\ \ \\\\\\\\\\\\\\\\\\\\\\\\\\\\ \\\\\ \
\\
‘
l.
Immune system of Cyprinid fish
439
cytotoxicity, mixed leucocyte reaction (MLR), macrophage migration in—
(MI) and antigen induced blastogenesis of lymphocytes.
‘
”
The kinetics of cellular immune reactions in cyprinid fish haVe been
ex—
"
studied by transplantations of scales and skin in goldfish and carp
The processes
involved in rejection have been reviewed by’ Hil—
’
(1970),
and
Botham et al.
(1980).
Although lymphocytes and mononu—
phagocytic cells are involved in rejection,- the
exact
meChanisms
not
well
understood e.g.
up
to
now
no
specific cytotoxic lymphocytes
”could be demonstrated.
At optimal temperatures allografts
were
always
rejected
in‘ an
acute
«
”manner,
the
medial
survival
time
(MST) less than 14 days (Rijkers, l982b).
example:
In goldfish et 30°C the
MST
of
first—
and
second—set
scale
were
7.2 and 4.7 days respectively.
No
significant antigen dosé effect
the MST of both first— and second—set allografts was
monitored.
‘
Moreover
‘secondary immune responses were already obtained 7 days after the first graft—
(Hildemann, 1970).
Rijkers (1982b) concluded that in the allograft rejec—
of
teleosts
most
immunological characteristics are comparable to those
'fbund in mammals, with
the
most
important
feature:_
the
formation
of
a
donor specific immunological memory.
‘
The in vitro test for histo—incompatibility, the MLR has
beendescribed
f°r“ carp (—Ca”spi—â Avtalion, 1984;
Gloudemàns et al-, 1985) -
It” Prdvéd *an“afPt
“method for MHC typing of carp (Gloudemans et al., 1985) and for
Studying- the
effect
(Grondel et al., 1985):‘ Grondel &
showed that in a MLR of carp pronephric leucocytes,
”lymphodyte
’growth
promoting
factors
releaSed into
the culture mediùm, re9èmbling
interleukin 2 (IL—2):
'
‘-"
No clear data are available on DTH réadtions in teleost
its
vitr0"manifestationg
test, in cyprinid
“The
ÎËÀ
“gâVe ”positive
g
‘et’
alé,
19825)'it
Was
shown that
fa6t0rs were inVQIVed
in
“this reaction;
for the supernatant of à M1 reaction by
con A, frdm Which
been'removed; Still had inhibitory
Antigen induced
lymph0cytes
been
in”
were?
bath against the pathogen
Although none of the fish developed
antibody,
natural
et
“Èàmers & Ideler
studied
from head kidney of vàCCînated
”ÎäÈëäïEÿ—îhé bacterial preparation,
fish were stimulated as “wgil,
faltoügh
that it Was a real manifestation
animals
to
A.
hydrophila
‘
of i1mùô
.
..
Jï‘t‘hat
E'?“Ë?ÈFÏÇWlËh; t_hÎ
añ—*
cas eof wpo’s
…,« ”indicates that
*ièrôïÿï‘f(°f tln>hbndV1pd
AS a1ready mentioned
“" I 980 ;
»' Ri“ jke r's‘ é tal
,
19 8056
,
\î Ï
,
_
Â
…
\\\
\\ \ \\\\\\\\\\\\\\\\\\\\\\\\\\\\ \\\\\ \
\\