210
Aquaculture of Cyprinids
by hypophysation. Rohu—catla, rohu—mrigal, catla—mrigal, catla—calbasu,
catla—fimbriatus, catla—silver carp and grass carp—silver carp crosses and
reciprocal crossess yielded hybrids showing a better growth rate than one of
the
parents,
and some yielded more flesh tant either of the parents. Rohu—
common
carp and mrigal—common carp hybrids did not attain maturity in the
ponds and the chromosomal complements of the parent species point to the
possibility that they are triploid and sterile.
INDUCED
GYNOGENÈSIS BY RETENTÎON OF THE
SECOND POLAR BODY IN THE COMMON CARP,
Cyprinus carpio L., AND HETEROZYGOSÎTY OF GYNOGENETIC PROGENY AT TRANSFERRIN
AND Ldh B1
LOCI.
O.
LÏNHAÊT, P. KVÀSNÏCKÀ, V. SLECHTOUÀ, and J. POKORNV.
FLAhQÆLQÆ RcA@aäch
CS—389 25
U0dmany, CZECHOSLOUÀKÏÀ.
Experiments on induced gynogenesis by the retention and fusion of the second
polar body were carried bout in common carp for 2 years (1983,1984). Gamma
rays
(ÔOCo; absorbed dose 1,000 Gy) were used to inactivate sperm DNA. To
reconstitute the diploid, the eggs were cold—shocked at 0—4°C for 60 min,
5
and
15 min after
the
spermatozoa and eggs were activated by the fertiliza—
tion solution. The gynogenetic nature of the progeny (i.e. the absence of
a
paternal genome) was tested at the loci for scaliness, transferrin and
LDH B1 phenotypes. The extensive variability of the ”mirror” maternal genoty—
pe was
observed in the
Offspring : phenotypes from ”mirror", "line", to
exclusively "nude” were recorded. Progeny heterozygosity at the transferrin
locus was
0,952 (n= 1 female) and 5.56% (n = 4 females) in 1983 and 1984,
respectively. Progeny heterozygosity at the Ldh B1
locus was
38,84 % (n =
2
females) and 42,422 (n = 2 females) in 1984 and 1984, respectively.
POLYPLOID MOSAICS
INDUCED IN ROHU, Labeo rohita (HAM). P.V.G.K. REÜÜV, G.
JOHN, cmd R.K. JANA. Fäex5hœætcä AquacuÏÎu/Ïcîäîæz,
O…ACL State, 751
BlwbanQAWM—75îooz, INDIA
Polyploid mosaics could be induced in rohu, Labeo rohita (Ham) for the first
time using colchicine. The concentration of colchicine solution used was
0.01%. The fertilized eggs were divided into three groups, A1, A2, and BGroups A]
and A
were
treated with
colchicine and group B was maintained
as
control.
ln
the
treatment was
given 4 min after fertilization, lasting
ill yolk invagination stage. The eggs of group A2 were treated 25 min after
fertilization, i.e. before the initiation of cleavage and lasting till
morula—stage. Mosaics were observed in group A2 only.
GENOME
MANIPULATION IN CARP
(Cyprinus carpio L.).(J. CHINGJIANG, V. VUZHEN
and C. RONGDE, ImtÇtuxïc 05 HyÆÏ06LOÆ09%
Wuhan P.R.
CHINA.
An attempt was made to learn the potential applications of genome manipula—
tion
in fish breeding. Gynogenetic diploid and triploid carp were produced
by haploid manipulation of the genome. The haploid is favorable material
for karyotype analysis. Karyotype analysis of the haploid showed that the
chromosome complement had : 6 metacentrics (M), 20 submetacentrics (SM) and
24 subtelocentrics
or
telocentrics
(ST or T). Karyotype analysis demonstrated that the
chromosome
complements of the gynogenetic diploid were from two
complete genomes with normal viability and a functional ovary : combining
gynogenesis with artificial sex reversal, the second gynogenetic generation
could be employed as a stock for establishing a pure line. The chromosome
complement
of
some
triploid individuals with normal functional ovaries
con51sted of
three
complete genomes
(euploid).
Aquaculture of Cyprinids
by hypophysation. Rohu—catla, rohu—mrigal, catla—mrigal, catla—calbasu,
catla—fimbriatus, catla—silver carp and grass carp—silver carp crosses and
reciprocal crossess yielded hybrids showing a better growth rate than one of
the
parents,
and some yielded more flesh tant either of the parents. Rohu—
common
carp and mrigal—common carp hybrids did not attain maturity in the
ponds and the chromosomal complements of the parent species point to the
possibility that they are triploid and sterile.
INDUCED
GYNOGENÈSIS BY RETENTÎON OF THE
SECOND POLAR BODY IN THE COMMON CARP,
Cyprinus carpio L., AND HETEROZYGOSÎTY OF GYNOGENETIC PROGENY AT TRANSFERRIN
AND Ldh B1
LOCI.
O.
LÏNHAÊT, P. KVÀSNÏCKÀ, V. SLECHTOUÀ, and J. POKORNV.
FLAhQÆLQÆ RcA@aäch
CS—389 25
U0dmany, CZECHOSLOUÀKÏÀ.
Experiments on induced gynogenesis by the retention and fusion of the second
polar body were carried bout in common carp for 2 years (1983,1984). Gamma
rays
(ÔOCo; absorbed dose 1,000 Gy) were used to inactivate sperm DNA. To
reconstitute the diploid, the eggs were cold—shocked at 0—4°C for 60 min,
5
and
15 min after
the
spermatozoa and eggs were activated by the fertiliza—
tion solution. The gynogenetic nature of the progeny (i.e. the absence of
a
paternal genome) was tested at the loci for scaliness, transferrin and
LDH B1 phenotypes. The extensive variability of the ”mirror” maternal genoty—
pe was
observed in the
Offspring : phenotypes from ”mirror", "line", to
exclusively "nude” were recorded. Progeny heterozygosity at the transferrin
locus was
0,952 (n= 1 female) and 5.56% (n = 4 females) in 1983 and 1984,
respectively. Progeny heterozygosity at the Ldh B1
locus was
38,84 % (n =
2
females) and 42,422 (n = 2 females) in 1984 and 1984, respectively.
POLYPLOID MOSAICS
INDUCED IN ROHU, Labeo rohita (HAM). P.V.G.K. REÜÜV, G.
JOHN, cmd R.K. JANA. Fäex5hœætcä AquacuÏÎu/Ïcîäîæz,
O…ACL State, 751
BlwbanQAWM—75îooz, INDIA
Polyploid mosaics could be induced in rohu, Labeo rohita (Ham) for the first
time using colchicine. The concentration of colchicine solution used was
0.01%. The fertilized eggs were divided into three groups, A1, A2, and BGroups A]
and A
were
treated with
colchicine and group B was maintained
as
control.
ln
the
treatment was
given 4 min after fertilization, lasting
ill yolk invagination stage. The eggs of group A2 were treated 25 min after
fertilization, i.e. before the initiation of cleavage and lasting till
morula—stage. Mosaics were observed in group A2 only.
GENOME
MANIPULATION IN CARP
(Cyprinus carpio L.).(J. CHINGJIANG, V. VUZHEN
and C. RONGDE, ImtÇtuxïc 05 HyÆÏ06LOÆ09%
Wuhan P.R.
CHINA.
An attempt was made to learn the potential applications of genome manipula—
tion
in fish breeding. Gynogenetic diploid and triploid carp were produced
by haploid manipulation of the genome. The haploid is favorable material
for karyotype analysis. Karyotype analysis of the haploid showed that the
chromosome complement had : 6 metacentrics (M), 20 submetacentrics (SM) and
24 subtelocentrics
or
telocentrics
(ST or T). Karyotype analysis demonstrated that the
chromosome
complements of the gynogenetic diploid were from two
complete genomes with normal viability and a functional ovary : combining
gynogenesis with artificial sex reversal, the second gynogenetic generation
could be employed as a stock for establishing a pure line. The chromosome
complement
of
some
triploid individuals with normal functional ovaries
con51sted of
three
complete genomes
(euploid).