R. Billard et J. Marcel, Ed. Aquaculture of Cyprinids, INRA, Paris, 1986.
summanes
DIPLOID
GYNOGENESIS INDUCED BY HEAT SHOCK AFTER FERTILTZATTON WITH THE U.V."
IRRADIED
SPERM OF
COMMON CARP.
M.G. HOLLEBECQ, D. CHOURROUT, and R. BILLARD,
ÏNRA, Dêpa&icmcnä
78350 JOUY EN JOSAS, FRANCE.
To
define a technique for inducing diploid gynogenesis in common carp, trials
to
optimize retention of the 2nd polar body were carried out by submitting
the eggs
to heat
shortly after they had been activated by U.V.—irradia—
ted sperm
(4 mW/cm ). Batches of about ZOO—300 ova were inseminated in fresh—
water
in Petri dishes
(@ :_5 cm), which were then transferred into a thermo—
regulated waterbath for heat shock. The fertilized eggs and embryos were
counted after
2 days and normal and abnormal larvae at 4 days. The following
optimum results were recorded : l) age at shock : 3, 4 and 5 min. (intervals
tested
:
between 30 sec and 30 min after activation); 2) shock temperature :
39—39.5—40°C (positive results : between 38 and 40°C for temperature tests
between 35 and 40°C; 3) shock duration (depending on temperature) : 2—3 min
at
38°C, 2 min at 39°C, 1.5—2 min at 39.5°C and 1.5 min at 40°C. The present
hatehing rate of viable gynogenetic diploid larvae compared to the initial
number of eggs
treated did not exceed 40 to 50%, a value that certainly
underestimates
the
frequency of the induced retention of the 2nd polar body.
The
gynogenetic transmission was checked by using a visible genetic marker
(red color).
ARTIFICIAL GYNOGENESIS IN THE INDIAN MAJOR CARP, Cirrhinus mrigala (Ham)
G. JOHN, P.V.G.K. REÛDV, and R.K. JANÀ. FÆQÆhWŒZQÆ Aquacu£Éuäè RQôeaäch and
TmAMng Cew;(ÿœ (CIFRI), P.O. KauAa£yagang, Bhubanexswær, 0…Aa, 757—002,
INDIA.
Artificial induction of gynogenesis is being reported for the first time in
mrigal, Cirrhinus mrigala (Ham). Mrigal eggs were activated by Cyprinus
carpio milt which had been irradiated by ultraviolet light. Diploidy in the
activated mrigal eggs was restored by cold shocking at 12°C for 10 min or
heat shocking at 30°C for 1 min. Heat shock appeared more effective in
restoring diploidy.
CURRENT STATUS OF HYBRIDTZATTON AMONGST'CULTTVATED CARPS IN INDIA. G.V.
KOWTAL, F.A.R.T.C., Ccmûa£ FÆ/5hQÆCQA, DhauLé, P.O. - KauAcŒyagang,
Bhubanuwm 757002, INDIA.
Indian carps, represented by catla, Catla catla;
rohu, Labeo rohita ; mrigal,
Cirrhinus mrigala; calbasu, Labeo calbaSU, Chinese carps
Silver
CâfP;
molitrix; grass carp, Ctenopharyngodon 1della
and European
play a vital role in enhanc1ng pond fish production.
These EËE—Eîgñiÿ_Eëäëàtible species : catla
and sllver CâïP are known
for
their faster growth rate, grass carp for its eff1c1ent weed—controlling
capabilites and common carp for its pond breed1ng and omn1vorous food habits;
all Indian carp are esteemed for their excellent quality fleSh and flavour.
Soon after the successful hypophysation Of carps
CUttaCk
(1957)
Worked on producing hybrids with desirable qual1t1es like
faster grogtË râte
and higher flesh yield. More than 36 interspec1fic
and
Ybîl_s d
Were
produced and many were found to be fertile; F2 generations were o
a1ne
summanes
DIPLOID
GYNOGENESIS INDUCED BY HEAT SHOCK AFTER FERTILTZATTON WITH THE U.V."
IRRADIED
SPERM OF
COMMON CARP.
M.G. HOLLEBECQ, D. CHOURROUT, and R. BILLARD,
ÏNRA, Dêpa&icmcnä
78350 JOUY EN JOSAS, FRANCE.
To
define a technique for inducing diploid gynogenesis in common carp, trials
to
optimize retention of the 2nd polar body were carried out by submitting
the eggs
to heat
shortly after they had been activated by U.V.—irradia—
ted sperm
(4 mW/cm ). Batches of about ZOO—300 ova were inseminated in fresh—
water
in Petri dishes
(@ :_5 cm), which were then transferred into a thermo—
regulated waterbath for heat shock. The fertilized eggs and embryos were
counted after
2 days and normal and abnormal larvae at 4 days. The following
optimum results were recorded : l) age at shock : 3, 4 and 5 min. (intervals
tested
:
between 30 sec and 30 min after activation); 2) shock temperature :
39—39.5—40°C (positive results : between 38 and 40°C for temperature tests
between 35 and 40°C; 3) shock duration (depending on temperature) : 2—3 min
at
38°C, 2 min at 39°C, 1.5—2 min at 39.5°C and 1.5 min at 40°C. The present
hatehing rate of viable gynogenetic diploid larvae compared to the initial
number of eggs
treated did not exceed 40 to 50%, a value that certainly
underestimates
the
frequency of the induced retention of the 2nd polar body.
The
gynogenetic transmission was checked by using a visible genetic marker
(red color).
ARTIFICIAL GYNOGENESIS IN THE INDIAN MAJOR CARP, Cirrhinus mrigala (Ham)
G. JOHN, P.V.G.K. REÛDV, and R.K. JANÀ. FÆQÆhWŒZQÆ Aquacu£Éuäè RQôeaäch and
TmAMng Cew;(ÿœ (CIFRI), P.O. KauAa£yagang, Bhubanexswær, 0…Aa, 757—002,
INDIA.
Artificial induction of gynogenesis is being reported for the first time in
mrigal, Cirrhinus mrigala (Ham). Mrigal eggs were activated by Cyprinus
carpio milt which had been irradiated by ultraviolet light. Diploidy in the
activated mrigal eggs was restored by cold shocking at 12°C for 10 min or
heat shocking at 30°C for 1 min. Heat shock appeared more effective in
restoring diploidy.
CURRENT STATUS OF HYBRIDTZATTON AMONGST'CULTTVATED CARPS IN INDIA. G.V.
KOWTAL, F.A.R.T.C., Ccmûa£ FÆ/5hQÆCQA, DhauLé, P.O. - KauAcŒyagang,
Bhubanuwm 757002, INDIA.
Indian carps, represented by catla, Catla catla;
rohu, Labeo rohita ; mrigal,
Cirrhinus mrigala; calbasu, Labeo calbaSU, Chinese carps
Silver
CâfP;
molitrix; grass carp, Ctenopharyngodon 1della
and European
play a vital role in enhanc1ng pond fish production.
These EËE—Eîgñiÿ_Eëäëàtible species : catla
and sllver CâïP are known
for
their faster growth rate, grass carp for its eff1c1ent weed—controlling
capabilites and common carp for its pond breed1ng and omn1vorous food habits;
all Indian carp are esteemed for their excellent quality fleSh and flavour.
Soon after the successful hypophysation Of carps
CUttaCk
(1957)
Worked on producing hybrids with desirable qual1t1es like
faster grogtË râte
and higher flesh yield. More than 36 interspec1fic
and
Ybîl_s d
Were
produced and many were found to be fertile; F2 generations were o
a1ne