Posîer summaries
1 71
where Ê;
2 were the constants of weight gain potential, feed maintenance
requirement and starvation weight loss, reSpectively; yo was the feed equi—
valent of natural production. The N of other species depended on x, accord—
ing to Walter's function : N = X (A + B 1 n X). The interspecific—effects
on
N were not revealed.
Except in S.c. egg production (E) was related to
individual weight (2) as E = a — %—, where a_and b_were constants. There was
no
correlation between body weight and egg production in S.c.
CHANGES
IN THE
CHORION DURING THE CORTTCAL REACTION IN CARP
(Cyprinus carpio
EGGS.
P.
RENARD, R. BÏLLARÛ, and R. CHRISTEN, INRA, Sïïi0n de PEyAÀOÆ09£Q
ÀWÂmŒKQ, 78350 JOUY EN JOSAS, FRANCE.
.
Changes in the chorion during the cortical reaction in carp eggs were
analysed using several techniques. 1) Kinetics of chorion swelling. After
unfertilized ova were immersed in fresh water or in a saline solution (S)
(150 mOsm/Kg), the chorion swelled, reaching a plateau within 12 or 18 min,
respectively. The same kinetic was observed when the ova were inseminated
before
they were immersed in these media. 2) Morphological analysis using
a
scanning electron microscope. During the cortical reaction in both ferti—
lized and unfertilized ova in water or in 8,
the outside of the surface of
the chorion, which was folded and presented pores, became practically smooth
within 10 min. Material accumulated gradually projected from the inner to
the outer part of the micropyle which was entirely clogged within 30 min.
3) Biochemical changes. Before the c0rtical reaction, the chorion was diges—
ted by the 6 proteases testes (pronase, proteinase K, trypsin, chymotrypsin,
collagenase dispase and elastase). As soon as the cortical reaction occured
and throughout embryogenesis, only proteinase K, pronase and elastase remain—
ed active. We tried to solubilize the chorion before or after the cortical
reaction in various
SDS and mercapto—ethanol—reducing media, but without
—
Success
because no protein was visualized on polyacrylamide gel. The bonds
of the constituent proteins seemed to be covalent as in the case of sea—
urchin eggs after hardening. 4) Profile of ovum fertilizability during the
cortical reaction. After the ova were diluted in water or in 8, their ferti—
lizability decreased from 90 to 50% in 1 or 9 min, respectively.
AMOUNT OF COLLECTED SPERM RELATED TO SPERMATOGENETIC PRODUCTION AND THE
POSSIBILITY OF SHORT—TERM STORAGE IN CARP. A. SAAD, and R. BILLARD, îNRA,
DepMemwï d‘Hydwbrorogœ, 78550 Jouy en Jaws, FRANCE.
Sperm production of the common carp (Cyprinus carpio) was estimated. Eight
males were killed at the end of the
1st sperm cy31e;
the number of sperma—
tozoa measured in their testes was
1.9 1 0.2
10
spz/Kg/year. The fraction
of this yield that could be used for artificial insemination was determined
after stimulating spermiation by injecting carp pituitary extract (CPE)When this treatment was carried out every week for one year,
96 Î 0-32 Of the
SP€rm production was collected. Ater one injection, sperm was collected 12 h
later and this amount was maximal 24 h after the injection. Sperm harvest
was
less when collection took place 48 or 96 h after injection. The response
increased—with the dose of cpe injected. In all cases, the fert11121ng abi—
1ity of sperm from treated males was equal to that of untreated males. Whole
SP6rm With 50 mg/l of penistreptomycin
added and stored at
4 C reta1ned
lts
fertilizing ability for 10 days. After dilution in a sa11ne solution
(50 mM
Of NaCl, pH 8, Tris buffer), the duration and intenSltÿ
Of mot111ty as WE11
as
fertilizing ability were markedly higher than in fresh water.
1 71
where Ê;
2 were the constants of weight gain potential, feed maintenance
requirement and starvation weight loss, reSpectively; yo was the feed equi—
valent of natural production. The N of other species depended on x, accord—
ing to Walter's function : N = X (A + B 1 n X). The interspecific—effects
on
N were not revealed.
Except in S.c. egg production (E) was related to
individual weight (2) as E = a — %—, where a_and b_were constants. There was
no
correlation between body weight and egg production in S.c.
CHANGES
IN THE
CHORION DURING THE CORTTCAL REACTION IN CARP
(Cyprinus carpio
EGGS.
P.
RENARD, R. BÏLLARÛ, and R. CHRISTEN, INRA, Sïïi0n de PEyAÀOÆ09£Q
ÀWÂmŒKQ, 78350 JOUY EN JOSAS, FRANCE.
.
Changes in the chorion during the cortical reaction in carp eggs were
analysed using several techniques. 1) Kinetics of chorion swelling. After
unfertilized ova were immersed in fresh water or in a saline solution (S)
(150 mOsm/Kg), the chorion swelled, reaching a plateau within 12 or 18 min,
respectively. The same kinetic was observed when the ova were inseminated
before
they were immersed in these media. 2) Morphological analysis using
a
scanning electron microscope. During the cortical reaction in both ferti—
lized and unfertilized ova in water or in 8,
the outside of the surface of
the chorion, which was folded and presented pores, became practically smooth
within 10 min. Material accumulated gradually projected from the inner to
the outer part of the micropyle which was entirely clogged within 30 min.
3) Biochemical changes. Before the c0rtical reaction, the chorion was diges—
ted by the 6 proteases testes (pronase, proteinase K, trypsin, chymotrypsin,
collagenase dispase and elastase). As soon as the cortical reaction occured
and throughout embryogenesis, only proteinase K, pronase and elastase remain—
ed active. We tried to solubilize the chorion before or after the cortical
reaction in various
SDS and mercapto—ethanol—reducing media, but without
—
Success
because no protein was visualized on polyacrylamide gel. The bonds
of the constituent proteins seemed to be covalent as in the case of sea—
urchin eggs after hardening. 4) Profile of ovum fertilizability during the
cortical reaction. After the ova were diluted in water or in 8, their ferti—
lizability decreased from 90 to 50% in 1 or 9 min, respectively.
AMOUNT OF COLLECTED SPERM RELATED TO SPERMATOGENETIC PRODUCTION AND THE
POSSIBILITY OF SHORT—TERM STORAGE IN CARP. A. SAAD, and R. BILLARD, îNRA,
DepMemwï d‘Hydwbrorogœ, 78550 Jouy en Jaws, FRANCE.
Sperm production of the common carp (Cyprinus carpio) was estimated. Eight
males were killed at the end of the
1st sperm cy31e;
the number of sperma—
tozoa measured in their testes was
1.9 1 0.2
10
spz/Kg/year. The fraction
of this yield that could be used for artificial insemination was determined
after stimulating spermiation by injecting carp pituitary extract (CPE)When this treatment was carried out every week for one year,
96 Î 0-32 Of the
SP€rm production was collected. Ater one injection, sperm was collected 12 h
later and this amount was maximal 24 h after the injection. Sperm harvest
was
less when collection took place 48 or 96 h after injection. The response
increased—with the dose of cpe injected. In all cases, the fert11121ng abi—
1ity of sperm from treated males was equal to that of untreated males. Whole
SP6rm With 50 mg/l of penistreptomycin
added and stored at
4 C reta1ned
lts
fertilizing ability for 10 days. After dilution in a sa11ne solution
(50 mM
Of NaCl, pH 8, Tris buffer), the duration and intenSltÿ
Of mot111ty as WE11
as
fertilizing ability were markedly higher than in fresh water.