Çonîmi of ovulation
145
PIM
together
With
had higher serum GtH levels at 8 hours than fish
receiv1ng
the
combination of 001 or l«0 mg/kg PIM and LHRH—A°
By 24 hours,
6
of
8
fish had
ovulated
in
response
to
LHRH—A alone°
Although PIM did not
potentiate the efrect of LHRH—A on ovulation, injection of 10 mg/kg PIM
together with LHRH—A resulted in a significant ovulatory response in
8 bourse
80
wæë
-—
E
60
8
24
U?
C
hours
I
E
'"
40
ID
Ü
::
7°‘5î.«
…‘5;
20
@
«‘;—%
…
*”
……
»_—3
;
0
====n
==1……
Ëä
Ëä
ËÊ
Veh
Pim
Pim
Pim
Veh
Pim
Pim
Pim
03
1
10
OJ
1
10
Ps
PS
PS
PS
LHRHA
LHRHA
LHRHA
LHRHA
0VULATION
%
%
%
%
% %
1/8 %
2/53 %
% %
Figure 3°
Effects
of
different dosages of PIM (mg/kg) and 0°1 mg/kg LHRH—A
on
serum
GtH
levels and ovulation in bream held at 22—24°C.
Serum GtH
levels are reported as the mean Î_SE°
' Significant1y greater than VEH—PS and PIM—PS groups at 8 and 24 hours
(P < 0.05)…
D Significantly greater than VEH—LHRH—A gr0up at 8 and 24 hours.
A Significantly greater than PIM 0.1—LHRH—A and PIM l…O—LHRH—A groups at
8 hours°
In a subsequent study, injections of an intermediate dosage of PIM
mg/kg) alone and a low dosage of LHRH-A (0.01 mg/kg) alone
were
hœffective in inducing ovulation in bream; simultaneous injection of PIM
LHRH—A at these doses caused ovulation in all treated fish Wlthln
24 h0urs (Table 4).
Injections of PIM together with a high dosage of LHRH—A
mg/kg) caused ovulation in 5 of 8 fish within 8 hours; no ovulati©ns
xœre
f0und in fish injected with LHRH—A alone at 8 hours post—injection.
HOWever by 24 hour5, injection of 0=1 mg/kg LHRH—A alone caused ovulation in
4 of 8 fish.