144
H.Ë. Lin etai.
Table 2.
Effects
of
length of time interval between injection of
pimozide (10 mg/kg) and LHRH—A (0.1 mg/kg) on ovulation in common
carp held at 20—22°C.
__________._______________________________________________________.
First
Second
Injection
Injection
Fish
Time
Treatment
Time
Treatment
N
Ovulated
___________________________________________________________________
13:00
VEH
19:00
PS
6
0
13:00
PIM
19:00
PS
9
0
13200
VEH
19:00
LHRH—A
6
0
09:00
PIM
19:00
LHRH—A
8
2
13:00
PIM
19:00
LHRH—A
8
5*
16:00
PIM
19:00
LHRH—A
9
6*
19:00
PIM
19:00
LHRH—A
9
7*
____________________________________________________________________
*
significantly greater than VEH—PS, PIM—PS and VEH—LHRH—A groups
(P < 0.05).
Silver Carp
The
effects
of PIM and LHRH—A on ovulation in silver carp are shown in
Table 3.
The
ovulatory response to injections of PIM alone and LHRH—A alone
did not
differ
from control fish.
The
combined
treatment
of PIM and LHRH-A
was
highly effective, causing ovulation within 12—16 hours°
Table 3.
Effects
of PIM
(10 mg/kg) and LHRH—A
(0.1 mg/kg) on ovulation in silver carp held at
21—23.5°C.
Fish
Response Time to
Treatment
N
Ovulated
Ovulation
(hours)
VEH
PS
6
0
___
PIM
PS
7
0
———
VEH
LHRH—A
7
3
24—30
PIM
LHRH—A
9
9*
12—16
*
significantly greater than other treatment groups
(P < 0.05).
Ê£ÊËE
The
results
of an initial experiment testing the effects Of graded
amounts
of PIM administered together with 0.1 mg/kg LHRH—A on serum GtH
levels and ovulation in bream are shown in Fig. 3.
Serum GtH
levels in
LHRH—A injected fish were elevated at both 8 and 24 hours when compared to
control
or
PIM—injected groups.
However, injection of PIM (0.1, 1 and
together with LHRH—A resulted in higher serum GtH levels than
injection of LHRH—A alone at both 8 and 24 hours.
Fish receiving 10 mg/kg