Fig. 3.5 DNA amplification through the PCR process. The PCR procedure has three steps, DNA
strands are (1) are separated by heating, then (2) annealed to an excess of short synthetic DNA
primers that flank the region to be amplified; (3) new DNA is synthesized by polymerization. The
three steps are repeated for 25 or 30 cycles. The thermostable DNA polymerase Taql (from
Thermus aquatics, a bacterial species that grows in hot springs) is not denatured by the heating
steps
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3 Fish Genetics
strands are (1) are separated by heating, then (2) annealed to an excess of short synthetic DNA
primers that flank the region to be amplified; (3) new DNA is synthesized by polymerization. The
three steps are repeated for 25 or 30 cycles. The thermostable DNA polymerase Taql (from
Thermus aquatics, a bacterial species that grows in hot springs) is not denatured by the heating
steps
62
3 Fish Genetics