423
15 FtIR Imaging of tissues: techniques and methods of Analysis
Table 15.1 Assignment of FtIR bands of cells and tissues
vibrational mode
Wavenumber (cm
−1
) Assignment
Nh stretch (amide A)
ca. 3290
Proteins
Nh stretch (amide B)
3060
Proteins
= Ch olefinic stretch
3010–3030
unsaturated lipids
Ch 3 asymmetric stretch
2954–2962
Lipids and protein
Ch 2 asymmetric stretch
2915–2924
Saturated lipids and side chain of
proteins
Ch 3 symmetric stretch
2870
Lipids and protein
Ch 2 symmetric stretch
2847–2855
Saturated lipids and side chain of
proteins
Sh stretch
2550
Cysteine thiol group
C = o stretch (ester)
1740–1730
Lipids, phospholipids
C = o stretch
1705–1690
Bases of nucleic acids
C = o stretch + Nh bend (amide I)
1695–1670
Proteins—anti-parallel β-sheet
and β-turn structures
1670–1660
Proteins—3 10 -helix structure
1660–1648
Proteins—α-helix structure
1637–1623
Proteins—β-sheet structure
ring CC stretch of tyr and Phe residues, and nucleotides
1620
Proteins, nucleic acids
Nh bend + C-N stretch (amide II)
1580–1480
Proteins
ring CC stretch of tyr residues
1520
tyrosine proteins
Ch 2 scissoring mode
1473–1462
Lipids proteins,
Ch 2 , Ch3 deformation modes
1455
Proteins
Coo
−
symmetric stretch
1400
Fatty acids, amino acids
Ch 3 deformation of aliphatic side
groups of amino acid residues
1379
Proteins
Ch 2 wagging
1343
Phospholipids, fatty acids, triglycerides, amino acid side chains
Amide III
1340–1240
Proteins
Po 2
−
asymmetric stretch
1238
dNA
Co-o-C asymmetric stratech
1171
Cholesteryl esters
C-oh stretch
1171
Seronine, thyrosine, threonine
C-o stretch
1151
glycogen, mucin
C-o stretch of ribose ring
1120
RNA
Po 2
−
symmetric stretch
1083–1086
Nucleic acids, phospholipids
C-C stretch
1078
glycogen
-Co-o-C stretches
1063
Cholesterol esters, phospholipids
Coh deformation
1050–1056
mucin, carbohydrates
1030
Nucleic acids
1022–1028
glycogen, carbohydrates
dianionic phosphate monoester
970
Phosphorylated proteins
C-N-C stretch of ribose-phosphate
skeletal vibrations
965
Nucleic acids
933
Z type dNA
15 FtIR Imaging of tissues: techniques and methods of Analysis
Table 15.1 Assignment of FtIR bands of cells and tissues
vibrational mode
Wavenumber (cm
−1
) Assignment
Nh stretch (amide A)
ca. 3290
Proteins
Nh stretch (amide B)
3060
Proteins
= Ch olefinic stretch
3010–3030
unsaturated lipids
Ch 3 asymmetric stretch
2954–2962
Lipids and protein
Ch 2 asymmetric stretch
2915–2924
Saturated lipids and side chain of
proteins
Ch 3 symmetric stretch
2870
Lipids and protein
Ch 2 symmetric stretch
2847–2855
Saturated lipids and side chain of
proteins
Sh stretch
2550
Cysteine thiol group
C = o stretch (ester)
1740–1730
Lipids, phospholipids
C = o stretch
1705–1690
Bases of nucleic acids
C = o stretch + Nh bend (amide I)
1695–1670
Proteins—anti-parallel β-sheet
and β-turn structures
1670–1660
Proteins—3 10 -helix structure
1660–1648
Proteins—α-helix structure
1637–1623
Proteins—β-sheet structure
ring CC stretch of tyr and Phe residues, and nucleotides
1620
Proteins, nucleic acids
Nh bend + C-N stretch (amide II)
1580–1480
Proteins
ring CC stretch of tyr residues
1520
tyrosine proteins
Ch 2 scissoring mode
1473–1462
Lipids proteins,
Ch 2 , Ch3 deformation modes
1455
Proteins
Coo
−
symmetric stretch
1400
Fatty acids, amino acids
Ch 3 deformation of aliphatic side
groups of amino acid residues
1379
Proteins
Ch 2 wagging
1343
Phospholipids, fatty acids, triglycerides, amino acid side chains
Amide III
1340–1240
Proteins
Po 2
−
asymmetric stretch
1238
dNA
Co-o-C asymmetric stratech
1171
Cholesteryl esters
C-oh stretch
1171
Seronine, thyrosine, threonine
C-o stretch
1151
glycogen, mucin
C-o stretch of ribose ring
1120
RNA
Po 2
−
symmetric stretch
1083–1086
Nucleic acids, phospholipids
C-C stretch
1078
glycogen
-Co-o-C stretches
1063
Cholesterol esters, phospholipids
Coh deformation
1050–1056
mucin, carbohydrates
1030
Nucleic acids
1022–1028
glycogen, carbohydrates
dianionic phosphate monoester
970
Phosphorylated proteins
C-N-C stretch of ribose-phosphate
skeletal vibrations
965
Nucleic acids
933
Z type dNA