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H. J. Byrne et al.
•  Increase in nucleic acid levels with increasing number of HR-HPV copies in the 
cell or increasing p16INK4A expression (Fig. 13.8a, d),
•  Decrease in lipid levels with increasing number of HR-HPV copies in the cell or 
increasing p16INK4A expression (Fig. 13.8b, e),
•  No tendency for changes in protein levels related to HR-HPV copy number or 
increasing p16INK4A expression (Fig. 13.8c, f).
the observed increase in nucleic acid levels may be related to an increased number
of chromosomes present in hPv infected nuclei. It was reported by mehés et al. that
hPv presence facilitates polyploidisation (increase in chromosome number in a cell
Fig. 13.8 Peak intensity analysis for FtIR spectra of nuclear and cytoplasmic regions of cervical
cancer cells. dependence of peak intensities vs. hPv copy number was fit with y = a + bIx + cx
function, while peak intensities vs. fluorescence intensities (p16INK4A expression level) was fit
with a linear function, y = a + bx. (Reproduced from [64])