325
12. Reddy RK, Bhargava R (2010) Chemometric methods or Biomedical Raman Spectroscopy
and Imaging. In: matousek P (ed) Emerging Raman applications and techniques in biomedical and pharmaceutical fields. Springer Berlin heidelberg, p 179–213
13. heraud P, Wood BR, Beardall J et al (2006) Effects of pre-processing of Raman spectra on in
vivo classification of nutrient status of microalgal cells. J Chemom 20:193–197
14. heraud P, Beardall J, mcNaughton d et al (2007) In vivo prediction of the nutrient status of
individual microalgal cells using Raman microspectroscopy. FEmS microbiol Lett 275:24–
30
15. Abbas A, Josefson m, Abrahamsson K (2011) Characterization and mapping of carotenoids
in the algae Dunaliella and Phaeodactylum using Raman and target orthogonal partial least
squares. Chemometr Intell Lab 107:174–177
16. milledge JJ (2011) Commercial application of microalgae other than as biofuels: a brief
review. Rev Environ Sci Biotechnol 10:31–41
17. Brennan L, owende P (2010) Biofuels from microalgae—a review of technologies for production, processing, and extractions of biofuels and co-products. Renev Sust Energ Rev
14:557–577
18. Spolaore P, Joannis-Cassan C, duran E et al (2006) Commercial applications of microalgae.
J Biosci Bioeng 101:87–96
19. Collins Am, Jones hd, han d et al (2011) Carotenoid distribution in living cells of Haematococcus pluvialis (Chlorophyceae). PLoS one 6:e24302
20. tao Z, Wang g, Xu X et al (2011) monitoring and rapid quantification of total carotenoids in
Rhodotorula glutinis cells using laser tweezers Raman spectroscopy. FEmS microbiol Lett
314:42–48
21. Pilat Z, Bernatova S, Jezek J et al (2012) Raman microspectroscopy of algal lipid bodies:
beta-carotene quantification. J Appl Phycol 24:541–546
22. han R-m, Zhang J-P, Skibsted Lh (2012) Reaction dynamics of Flavonoids and Carotenoids
as Antioxidants. molecules 17:2140–2160
23. Wormit m, dreuw A (2007) Quantum chemical insights in energy dissipation and carotenoid
radical cation formation in light harvesting complexes. Phys Chem Chem Phys 9:2917–2931
24. Wormit m, dreuw A (2006) Carotenoid radical cation formation in Lh2 of purple bacteria: a
quantum chemical study. J Phys Chem 110:24200–24206
25. Walla PJ, Linden PA, hsu C-P et al (2000) Femtosecond dynamics of the forbidden carotenoid S1 state in light-harvesting complexes of purple bacteria observed after two-photon
excitation. Natl Acad Sci 97:10808–10813
26. Wang Y, hu X (2002) A quantum chemistry study of binding carotenoids in the bacterial
light-harvesting complexes. J Am Chem Soc 124:8445–8451
27. Neugebauer J, veldstra J, Buda F (2011) theoretical Spectroscopy of Astaxanthin in Crustacyanin Proteins: Absorption, Circular dichroism, and Nuclear magnetic Resonance. J Phys
Chem B 115:3216
28. van Wijk AA, Spaans A, uzunbajakava N et al (2005) Spectroscopy and quantum chemical
modeling reveal a predominant contribution of excitonic interactions to the bathochromic
shift in α-crustacyanin, the Blue carotenoprotein in the carapace of the lobster Homarus gammarus. J Am Chem Soc 127:1438–1445
29. Kaczor A, turnau K, Baranska m (2011) In situ Raman imaging of astaxanthin in a single
microalgal cell. Analyst 136:1109–1112
30. Kaczor A, Baranska m (2011) Structural Changes of Carotenoid Astaxanthin in a Single
Algal Cell monitored in Situ by Raman Spectroscopy. Anal Chem 83:7763–7770
31. Kubo Y, Ikeda t, Yang S-Y et al (2000) orientation of carotenoid molecules in the eyespot of
alga: In Situ polarized resonance Raman spectroscopy. Appl Spectrosc 54:1114–1119
32. urban PL, Schmid t, Amantonico A et al (2011) multidimensional analysis of single algal
cells by integrating microspectroscopy with mass spectrometry. Anal Chem 83:1843–1849
33. huang Y, Beal C, Cai W et al (2010) micro-Raman spectroscopy of algae: composition analysis and fluorescence background behavior. Biotechnol Bioeng 105:889–898
11 Structural and Spatial Analysis of Carotenoids in a Single Cell monitored …
12. Reddy RK, Bhargava R (2010) Chemometric methods or Biomedical Raman Spectroscopy
and Imaging. In: matousek P (ed) Emerging Raman applications and techniques in biomedical and pharmaceutical fields. Springer Berlin heidelberg, p 179–213
13. heraud P, Wood BR, Beardall J et al (2006) Effects of pre-processing of Raman spectra on in
vivo classification of nutrient status of microalgal cells. J Chemom 20:193–197
14. heraud P, Beardall J, mcNaughton d et al (2007) In vivo prediction of the nutrient status of
individual microalgal cells using Raman microspectroscopy. FEmS microbiol Lett 275:24–
30
15. Abbas A, Josefson m, Abrahamsson K (2011) Characterization and mapping of carotenoids
in the algae Dunaliella and Phaeodactylum using Raman and target orthogonal partial least
squares. Chemometr Intell Lab 107:174–177
16. milledge JJ (2011) Commercial application of microalgae other than as biofuels: a brief
review. Rev Environ Sci Biotechnol 10:31–41
17. Brennan L, owende P (2010) Biofuels from microalgae—a review of technologies for production, processing, and extractions of biofuels and co-products. Renev Sust Energ Rev
14:557–577
18. Spolaore P, Joannis-Cassan C, duran E et al (2006) Commercial applications of microalgae.
J Biosci Bioeng 101:87–96
19. Collins Am, Jones hd, han d et al (2011) Carotenoid distribution in living cells of Haematococcus pluvialis (Chlorophyceae). PLoS one 6:e24302
20. tao Z, Wang g, Xu X et al (2011) monitoring and rapid quantification of total carotenoids in
Rhodotorula glutinis cells using laser tweezers Raman spectroscopy. FEmS microbiol Lett
314:42–48
21. Pilat Z, Bernatova S, Jezek J et al (2012) Raman microspectroscopy of algal lipid bodies:
beta-carotene quantification. J Appl Phycol 24:541–546
22. han R-m, Zhang J-P, Skibsted Lh (2012) Reaction dynamics of Flavonoids and Carotenoids
as Antioxidants. molecules 17:2140–2160
23. Wormit m, dreuw A (2007) Quantum chemical insights in energy dissipation and carotenoid
radical cation formation in light harvesting complexes. Phys Chem Chem Phys 9:2917–2931
24. Wormit m, dreuw A (2006) Carotenoid radical cation formation in Lh2 of purple bacteria: a
quantum chemical study. J Phys Chem 110:24200–24206
25. Walla PJ, Linden PA, hsu C-P et al (2000) Femtosecond dynamics of the forbidden carotenoid S1 state in light-harvesting complexes of purple bacteria observed after two-photon
excitation. Natl Acad Sci 97:10808–10813
26. Wang Y, hu X (2002) A quantum chemistry study of binding carotenoids in the bacterial
light-harvesting complexes. J Am Chem Soc 124:8445–8451
27. Neugebauer J, veldstra J, Buda F (2011) theoretical Spectroscopy of Astaxanthin in Crustacyanin Proteins: Absorption, Circular dichroism, and Nuclear magnetic Resonance. J Phys
Chem B 115:3216
28. van Wijk AA, Spaans A, uzunbajakava N et al (2005) Spectroscopy and quantum chemical
modeling reveal a predominant contribution of excitonic interactions to the bathochromic
shift in α-crustacyanin, the Blue carotenoprotein in the carapace of the lobster Homarus gammarus. J Am Chem Soc 127:1438–1445
29. Kaczor A, turnau K, Baranska m (2011) In situ Raman imaging of astaxanthin in a single
microalgal cell. Analyst 136:1109–1112
30. Kaczor A, Baranska m (2011) Structural Changes of Carotenoid Astaxanthin in a Single
Algal Cell monitored in Situ by Raman Spectroscopy. Anal Chem 83:7763–7770
31. Kubo Y, Ikeda t, Yang S-Y et al (2000) orientation of carotenoid molecules in the eyespot of
alga: In Situ polarized resonance Raman spectroscopy. Appl Spectrosc 54:1114–1119
32. urban PL, Schmid t, Amantonico A et al (2011) multidimensional analysis of single algal
cells by integrating microspectroscopy with mass spectrometry. Anal Chem 83:1843–1849
33. huang Y, Beal C, Cai W et al (2010) micro-Raman spectroscopy of algae: composition analysis and fluorescence background behavior. Biotechnol Bioeng 105:889–898
11 Structural and Spatial Analysis of Carotenoids in a Single Cell monitored …