spectra. The details of the spectra will be discussed later. It is however obvious at first
glance that a strong dependence on the photon energy is observed.
Native trap content and photon flux are known parameters. In an action spectroscopy approach, the loss of precursor molecules from the trap can thus be used to
directly determine the relative total photoionisation cross section. Similarly, relative partial ion yields can be determined, which are directly linked to the total
photoionisation cross section.
Fig. 11.2 (a) Schematic structure of the polypeptide leucine enkephalin with amino acids
sequence tyrosine-glycine-glycine-phenylalanine-leucine (YGGFL). (b) VUV photofragmentation mass spectra of (YGGFL+H)
+ obtained at 8 eV, 15 eV and 20 eV [15]. Reprinted
with permission from [15]. Copyright 2011, AIP Publishing LLC
11 Action Spectroscopy of Gas-Phase Peptide Ions with Energetic Photons
213
glance that a strong dependence on the photon energy is observed.
Native trap content and photon flux are known parameters. In an action spectroscopy approach, the loss of precursor molecules from the trap can thus be used to
directly determine the relative total photoionisation cross section. Similarly, relative partial ion yields can be determined, which are directly linked to the total
photoionisation cross section.
Fig. 11.2 (a) Schematic structure of the polypeptide leucine enkephalin with amino acids
sequence tyrosine-glycine-glycine-phenylalanine-leucine (YGGFL). (b) VUV photofragmentation mass spectra of (YGGFL+H)
+ obtained at 8 eV, 15 eV and 20 eV [15]. Reprinted
with permission from [15]. Copyright 2011, AIP Publishing LLC
11 Action Spectroscopy of Gas-Phase Peptide Ions with Energetic Photons
213