5.14 Studies of Spectral Features of Radiation Scattered by Blood …
469
With the aim of developing a criterion for the quantitative estimation of concentration of oxyhemoglobin in blood and normalizing the results of measurements,
coefficients Q 1 and Q 2 were calculated that correspond to the ratio of amplitudes of
characteristic local extremums, of registered spectral curves at wavelengths of 542,
560, 577 nm and concentration of protein in the probes under study
Q 1 = Ka λ 1 − Ka λ 2 ; Q 2 = Ka λ 3 − Ka λ 2 ,
where Ka λ 1 = A 542 /B; Ka λ 2 = A 560 /B; Ka λ 3 = A 577 /B, where A 542 , A 560 , A 577 are
the amplitudes of spectral curve at wavelengths of 542, 560 and 577 nm; B is the
concentration of protein in probes under study.
The results of the measurements and calculations of coefficients Q 1 and Q 2 ,
suggested for the estimation of the degree of oxygenation of blood preparations,
are presented in Tables 5.7, 5.8 and 5.9.
On the basis of the experimental studies conducted with the help of threewavelength spectrophotometric method, it was found out that ILBI at traumatic
damages of PN in experimental animals leads to the change of 30 and 37%, respectively, to the calculated coefficients, which correspond to the difference between
amplitude ratio of local extremums of absorption spectra of native blood preparation
at wavelengths λ 1 = 542 nm, λ 2 = 560 nm and λ 3 = 577 nm to the protein concentration in the probes under study, in comparison with the analogous coefficients of
animals of the control group.
5.15 Studies of Spectral Properties of Radiation Scattered
by Blood Preparation of Animals at Partial Ischemia
of Sciatic Nerve Before and After ILBI with the Help
of Semiconductor Laser
The study was conducted on the spectral properties of radiation in the range of 200–
900 nm scattered by native blood preparations of experimental animals (rabbits)
at partial ischemia of sciatic nerve before and after the course of ILBI therapy
(semiconductor laser with λ = 850 nm).
The studies were conducted on blood preparation of 17 experimental animals.
With the help of spectrophotometer “Specord M-40,” absorption spectra of blood
preparation were registered, on which characteristic local extremums at wavelengths
of 542, 569 and 577 nm were recorded, the amplitude of which was determined by
the degree of blood oxygenation. The concentration of protein in the blood probes
under study was defined by biuretic method.
For the estimation of the influence of ILBI on the change of concentration of
oxyhemoglobin at partial ischemia of sciatic nerve, spectra of absorption of blood
preparation were studied and protein concentration was defined in the probes under
study of ten experimental animals (rabbits) before and after conducting the course
469
With the aim of developing a criterion for the quantitative estimation of concentration of oxyhemoglobin in blood and normalizing the results of measurements,
coefficients Q 1 and Q 2 were calculated that correspond to the ratio of amplitudes of
characteristic local extremums, of registered spectral curves at wavelengths of 542,
560, 577 nm and concentration of protein in the probes under study
Q 1 = Ka λ 1 − Ka λ 2 ; Q 2 = Ka λ 3 − Ka λ 2 ,
where Ka λ 1 = A 542 /B; Ka λ 2 = A 560 /B; Ka λ 3 = A 577 /B, where A 542 , A 560 , A 577 are
the amplitudes of spectral curve at wavelengths of 542, 560 and 577 nm; B is the
concentration of protein in probes under study.
The results of the measurements and calculations of coefficients Q 1 and Q 2 ,
suggested for the estimation of the degree of oxygenation of blood preparations,
are presented in Tables 5.7, 5.8 and 5.9.
On the basis of the experimental studies conducted with the help of threewavelength spectrophotometric method, it was found out that ILBI at traumatic
damages of PN in experimental animals leads to the change of 30 and 37%, respectively, to the calculated coefficients, which correspond to the difference between
amplitude ratio of local extremums of absorption spectra of native blood preparation
at wavelengths λ 1 = 542 nm, λ 2 = 560 nm and λ 3 = 577 nm to the protein concentration in the probes under study, in comparison with the analogous coefficients of
animals of the control group.
5.15 Studies of Spectral Properties of Radiation Scattered
by Blood Preparation of Animals at Partial Ischemia
of Sciatic Nerve Before and After ILBI with the Help
of Semiconductor Laser
The study was conducted on the spectral properties of radiation in the range of 200–
900 nm scattered by native blood preparations of experimental animals (rabbits)
at partial ischemia of sciatic nerve before and after the course of ILBI therapy
(semiconductor laser with λ = 850 nm).
The studies were conducted on blood preparation of 17 experimental animals.
With the help of spectrophotometer “Specord M-40,” absorption spectra of blood
preparation were registered, on which characteristic local extremums at wavelengths
of 542, 569 and 577 nm were recorded, the amplitude of which was determined by
the degree of blood oxygenation. The concentration of protein in the blood probes
under study was defined by biuretic method.
For the estimation of the influence of ILBI on the change of concentration of
oxyhemoglobin at partial ischemia of sciatic nerve, spectra of absorption of blood
preparation were studied and protein concentration was defined in the probes under
study of ten experimental animals (rabbits) before and after conducting the course