468
5 Laser Specklometer, Speckle-Optical Diagnostics and Laser …
transfer is due to the activity of biologically active compounds, the emission of which
into the bloodstream is stimulated by the activity of laser light. A change of the bioenergetics of red blood cell can be the consequence of the activity of such endogenous
bioregulators, the final result of which is higher level of oxygenation of endoglobular
hemoglobin in animals with ILBI that has been found out by the experiment.
Based on the results of the conducted work, it is possible to conclude that the
further development of the ideas about the mechanism of photochemical activation
of oxyhemoglobin compounds in condition in vivo can be reached in the studies with
the use of model systems with direct radiation of laser light as a structural form of
hemoglobin (solid red blood cell) and also the homogenous aqueous solution of this
protein.
5.14.2 Studies of Influence of ILBI on Absorption Spectra
of Blood Preparation of Animals with Traumatic
Damages of Peripheral Nerves During the Usage
of Radiation of He–Ne Laser
This paragraph is devoted to the study of spectral properties of radiation in a range of
200–900 nm scattered by native blood preparations of experimental animals during
traumatic damage of peripheral nerves (PN) before and after the course of ILBI
therapy (He–Ne laser λ = 632.8 nm).
The studies were conducted at blood preparations of nine experimental animals.
With the help of spectrophotometer “Specord M-40,” absorption spectra of blood
preparation were registered, on which characteristic local extremums were registered
at wavelengths of 542, 560 and 577 nm, the amplitude of which according to the
previously conducted studies was determined by the degree of blood oxygenation.
Protein concentration in the blood probes under study was determined by the biuretic
method.
The studies of absorption spectra of blood preparation were conducted for the
estimation of ILBI influence on the change of the concentration of oxyhemoglobin
at traumatic damages of sciatic nerve; also, the protein concentration in the probes
under study in nine experimental animals before and after conducting ILBI course
(10 sessions during 10 days) was determined. The exposure time amounted to 10
min at the power of radiation at the output of light guide equal to 2–2.5 mW.
ILBI course therapy at rabbits with the trauma of PN was started in 10 days after
operation. There was one course (10 procedures).
Spectral properties of radiation scattered by blood preparation of experimental
animals were studied in one month after conducting ILBI therapy.
Along with this, absorption spectra (1st and 2nd probes) were registered twice
and protein concentration in blood preparation in seven experimental animals, which
constitute control group, was determined. Animals with the damage of PN served as
control, and they did not undergo the course of ILBI therapy.
5 Laser Specklometer, Speckle-Optical Diagnostics and Laser …
transfer is due to the activity of biologically active compounds, the emission of which
into the bloodstream is stimulated by the activity of laser light. A change of the bioenergetics of red blood cell can be the consequence of the activity of such endogenous
bioregulators, the final result of which is higher level of oxygenation of endoglobular
hemoglobin in animals with ILBI that has been found out by the experiment.
Based on the results of the conducted work, it is possible to conclude that the
further development of the ideas about the mechanism of photochemical activation
of oxyhemoglobin compounds in condition in vivo can be reached in the studies with
the use of model systems with direct radiation of laser light as a structural form of
hemoglobin (solid red blood cell) and also the homogenous aqueous solution of this
protein.
5.14.2 Studies of Influence of ILBI on Absorption Spectra
of Blood Preparation of Animals with Traumatic
Damages of Peripheral Nerves During the Usage
of Radiation of He–Ne Laser
This paragraph is devoted to the study of spectral properties of radiation in a range of
200–900 nm scattered by native blood preparations of experimental animals during
traumatic damage of peripheral nerves (PN) before and after the course of ILBI
therapy (He–Ne laser λ = 632.8 nm).
The studies were conducted at blood preparations of nine experimental animals.
With the help of spectrophotometer “Specord M-40,” absorption spectra of blood
preparation were registered, on which characteristic local extremums were registered
at wavelengths of 542, 560 and 577 nm, the amplitude of which according to the
previously conducted studies was determined by the degree of blood oxygenation.
Protein concentration in the blood probes under study was determined by the biuretic
method.
The studies of absorption spectra of blood preparation were conducted for the
estimation of ILBI influence on the change of the concentration of oxyhemoglobin
at traumatic damages of sciatic nerve; also, the protein concentration in the probes
under study in nine experimental animals before and after conducting ILBI course
(10 sessions during 10 days) was determined. The exposure time amounted to 10
min at the power of radiation at the output of light guide equal to 2–2.5 mW.
ILBI course therapy at rabbits with the trauma of PN was started in 10 days after
operation. There was one course (10 procedures).
Spectral properties of radiation scattered by blood preparation of experimental
animals were studied in one month after conducting ILBI therapy.
Along with this, absorption spectra (1st and 2nd probes) were registered twice
and protein concentration in blood preparation in seven experimental animals, which
constitute control group, was determined. Animals with the damage of PN served as
control, and they did not undergo the course of ILBI therapy.