164
2 Holographic Microscopy of Phase and Diffuse Objects …
isolated nerve. In the case of the isolated nerve, the oppressive action of hyperbary
increased by the following decompression. Under hydrostatic pressure, the decrease
of nerve excitability was observed beginning from 160 atm. and had a deo-dependent
character. This effect had a reversible character: after full decompression, there was
observed a temporary recovery of excitability of the isolated nerve was observed.
The method of lymphoid cells cultivation was developed to obtain viable suspensions. There were conducted experiments in studying the lymphocytes functional
activity under antigens impact with the holographic interference microscopy method.
It was ascertained that the antigens impact leads to decrease of optical density of the
immune (pathologic) lymphocytes in comparison with the control (normal) lymphocytes. With the holographic interference microscopy, the results were obtained, which
gave possibility to determine the criterion for differentiation of the immune and
non-immune lymphocytes that can be of great interest for the applied medicine for
laboratory diagnostics improvement.
The hyperbary impact was tested for proliferative activity of animals lymphocytes
as well. It was ascertained that the proliferative activity in the spleen cells as well as
in the thymus ones increases under mitogens that testifies the intensification of the
organism response under high pressure.
3. There was developed and tested the holographic interference microscopy method
for thermal process study in laser diodes. In particular with its help, there
were studied in real time the resonator edge deformations of two-sided heterostructured injection lasers in the system under the pump current perlocation
(100–200 mA) and the cooling vessel heating in the range of temperature from
289 to 423 K. The measurement accuracy of laser diode edge deformation in the
direction of observation for interference fringe recording was 15–20 nm when
the absolute values of deformation were 1–2 μm. The application of the developed method made it possible to study the dependence of the relative velocity of
the heterolaser degradation on the thermoelastic voltage values. The holographic
research of edge deformations of the laser diode and the cooling edge under
heating of the latter made it possible to study the mechanical characteristics of
the connection of the system injection laser–indium solder–cooling vessel in the
temperature interval of 289–423 K.
To study the thermal process dynamics in pulse heterolasers (pump currents are
50–200 mA), a holographic method is developed, which makes it possible to conduct
measurements considering oscillation in the time of the zeroth band of the interference pattern. The holographic way of measurement with the accuracy of 2% of
thermal deformations and linear expansion coefficients of thin (5–10 μm) epitaxial
heterolaser layers was developed based on the multiple reflection of the object wave
from the surface of the layer under study and introduction of the calibrated phase
delay in the reference wave.
The two-channel holographic microinterferometry method was developed based
on the resonator sounding of the laser diode from the side of its opposite edges that
2 Holographic Microscopy of Phase and Diffuse Objects …
isolated nerve. In the case of the isolated nerve, the oppressive action of hyperbary
increased by the following decompression. Under hydrostatic pressure, the decrease
of nerve excitability was observed beginning from 160 atm. and had a deo-dependent
character. This effect had a reversible character: after full decompression, there was
observed a temporary recovery of excitability of the isolated nerve was observed.
The method of lymphoid cells cultivation was developed to obtain viable suspensions. There were conducted experiments in studying the lymphocytes functional
activity under antigens impact with the holographic interference microscopy method.
It was ascertained that the antigens impact leads to decrease of optical density of the
immune (pathologic) lymphocytes in comparison with the control (normal) lymphocytes. With the holographic interference microscopy, the results were obtained, which
gave possibility to determine the criterion for differentiation of the immune and
non-immune lymphocytes that can be of great interest for the applied medicine for
laboratory diagnostics improvement.
The hyperbary impact was tested for proliferative activity of animals lymphocytes
as well. It was ascertained that the proliferative activity in the spleen cells as well as
in the thymus ones increases under mitogens that testifies the intensification of the
organism response under high pressure.
3. There was developed and tested the holographic interference microscopy method
for thermal process study in laser diodes. In particular with its help, there
were studied in real time the resonator edge deformations of two-sided heterostructured injection lasers in the system under the pump current perlocation
(100–200 mA) and the cooling vessel heating in the range of temperature from
289 to 423 K. The measurement accuracy of laser diode edge deformation in the
direction of observation for interference fringe recording was 15–20 nm when
the absolute values of deformation were 1–2 μm. The application of the developed method made it possible to study the dependence of the relative velocity of
the heterolaser degradation on the thermoelastic voltage values. The holographic
research of edge deformations of the laser diode and the cooling edge under
heating of the latter made it possible to study the mechanical characteristics of
the connection of the system injection laser–indium solder–cooling vessel in the
temperature interval of 289–423 K.
To study the thermal process dynamics in pulse heterolasers (pump currents are
50–200 mA), a holographic method is developed, which makes it possible to conduct
measurements considering oscillation in the time of the zeroth band of the interference pattern. The holographic way of measurement with the accuracy of 2% of
thermal deformations and linear expansion coefficients of thin (5–10 μm) epitaxial
heterolaser layers was developed based on the multiple reflection of the object wave
from the surface of the layer under study and introduction of the calibrated phase
delay in the reference wave.
The two-channel holographic microinterferometry method was developed based
on the resonator sounding of the laser diode from the side of its opposite edges that