2.3 Holographic Study with Electrophysiological Control …
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refraction index and the shape of muscle fibers. However, it is necessary to gain a
certain amount of statistical data for getting information about what contributes more
to the phase incursion—the refraction index or the form of the muscle fibers.
Implementation of the holographic methods adapted to the studies of isolated
muscle fibers under gaseous hyperbary made it possible to get alteration of phase
incursion profiles in the muscle fiber from the interference measurements at pressure
application that points out the structural changes in muscle fibers.
2.4 Development and Improvement of the Holographic
Interference Microscopy Method for Studying
Deformations Occurred Under Thermal Heating
of Mirror and Diffusely Scattering Microobjects
An isolated nerve fiber, unlike compounding it microobjects such as an isolated nerve
fiber, a cell and muscle fiber in their lifetime, has a reflecting or a diffusing scattering
surface that significantly hampers and sometimes makes impossible its studies by
the traditional methods of optical microscopy.
It requires search and development of new methodological and technical ways,
which allow conducting studies with interferometric accuracy, for example, pulsation
amplitudes of a certain area of a muscle, nerve, shift value of their surface, changes of
their sizes during their vital functionality or under external factors. As such factors,
it is natural to observe temperature, environmental pressure and study their influence
on the neuromuscular fiber. Thus, it is known that under normal conditions of nerve
cell functioning lipids are in liquid-crystalline, partially ordered state and passes into
another crystal phase under decrease of temperature.
Phase transition under temperature change influences greatly biological processes
in a cell: Intensity and character of ion transport change sharply, kinetic characteristics of lipids change uppermost because of their viscosity that immediately
affects the protein diffusion coefficient. The study of a wide range temperature
impact (from low to high temperatures) is of great practical interest for the development of medical physiotherapy methods as well as for cryobiology (preservation of organs). Thus, for example, in the work [111] using holographic interference microscopy, optimal velocities of temperature decrease were ascertained, under
which erythrocytes preservation is provided and so on.
The holographic interferometry methods were approved during the study of
tympanic membrane vibrations. Alterations of vibrations frequency up to 5000 Hz
made it possible to detect imperfections in its structure. The data show the possibility and the appropriateness of conducting the study connected with the development and improvement of the holographic interference microscopy method to
analyze the neuromuscular tissue deformations, which occur under thermal heating.
But during the search of the microobject, which is optimal for methodological decisions processing at the stage of method development, a model system, namely, the
149
refraction index and the shape of muscle fibers. However, it is necessary to gain a
certain amount of statistical data for getting information about what contributes more
to the phase incursion—the refraction index or the form of the muscle fibers.
Implementation of the holographic methods adapted to the studies of isolated
muscle fibers under gaseous hyperbary made it possible to get alteration of phase
incursion profiles in the muscle fiber from the interference measurements at pressure
application that points out the structural changes in muscle fibers.
2.4 Development and Improvement of the Holographic
Interference Microscopy Method for Studying
Deformations Occurred Under Thermal Heating
of Mirror and Diffusely Scattering Microobjects
An isolated nerve fiber, unlike compounding it microobjects such as an isolated nerve
fiber, a cell and muscle fiber in their lifetime, has a reflecting or a diffusing scattering
surface that significantly hampers and sometimes makes impossible its studies by
the traditional methods of optical microscopy.
It requires search and development of new methodological and technical ways,
which allow conducting studies with interferometric accuracy, for example, pulsation
amplitudes of a certain area of a muscle, nerve, shift value of their surface, changes of
their sizes during their vital functionality or under external factors. As such factors,
it is natural to observe temperature, environmental pressure and study their influence
on the neuromuscular fiber. Thus, it is known that under normal conditions of nerve
cell functioning lipids are in liquid-crystalline, partially ordered state and passes into
another crystal phase under decrease of temperature.
Phase transition under temperature change influences greatly biological processes
in a cell: Intensity and character of ion transport change sharply, kinetic characteristics of lipids change uppermost because of their viscosity that immediately
affects the protein diffusion coefficient. The study of a wide range temperature
impact (from low to high temperatures) is of great practical interest for the development of medical physiotherapy methods as well as for cryobiology (preservation of organs). Thus, for example, in the work [111] using holographic interference microscopy, optimal velocities of temperature decrease were ascertained, under
which erythrocytes preservation is provided and so on.
The holographic interferometry methods were approved during the study of
tympanic membrane vibrations. Alterations of vibrations frequency up to 5000 Hz
made it possible to detect imperfections in its structure. The data show the possibility and the appropriateness of conducting the study connected with the development and improvement of the holographic interference microscopy method to
analyze the neuromuscular tissue deformations, which occur under thermal heating.
But during the search of the microobject, which is optimal for methodological decisions processing at the stage of method development, a model system, namely, the