126
2 Holographic Microscopy of Phase and Diffuse Objects …
Fig. 2.26 Interferogram of lymphocyte, derived with the help of holographic interference
microscope. Reprinted from [136] with permission
ϕ =
S
N d
, S =
N
i=1
x i ,
(2.35)
where S is the average phase incursion, which is carried into the object wave by
a lymphocyte; x i is the inclination of the interference wave in the i-point; N is the
number of measurements (the number of points, in which the inclination of the fringe
was measured); d is the interference pattern period.
The distributions of normal and immune lymphocytes according to the ϕparameter are presented in Fig. 2.27. Certain population heterogeneity emerges due
to this criterion. Thus, range ϕ is 0.029 when the most probable meanings are 0.31–
0.41 for lymphocytes with the diameter of 10 μm (normal and immune). An average
quadratic deviation is equal in both cases to δ = 0.05. If the limits of effective range
of the phase incursion carried into the object wave are the same (i.e., if the variations
Fig. 2.27 Distribution of regular (a) and immune (b) lymphocytes by parameter ϕ. Reprinted from
[136] with permission