100
2 Holographic Microscopy of Phase and Diffuse Objects …
Fig. 2.12 Sections of 3D images of the nerve fiber at overfocusing, reconstructed from one
hologram and varying on depth: a, b 3 μm, c–e 5 μm. Reprinted from [136] with permission
Depth of field (~5 μm) of reconstructed from a hologram image exceeds in 3–5
times the one at photography recording. These studies enabled to produce holographic
images of the main structural masses (myelin sheath, perikaryon of Schwann’s cell,
structure details of Ranvie interception).
The works [1–3, 5, 7–9, 15] are dedicated to the development and improvement
of holographic methods of lifetime study of nerve fibers using the regimes of their
rhythmical electrostimulation and single excitability action.
The chamber developed for these purposes (Fig. 2.13a) enabled to carry out holographic studies with simultaneous electrophysiological control (Fig. 2.13b) that made
it possible to follow the lifetime state of the nerve fiber as well as to control the process
of removal synchronization by both methods.
2.2.5 Influence of Magnetic Fields on Biological Objects
More often in clinical practice, magnetic fields are used, and moreover, there can be
used magnetic fields with constant and variable magnets in intensity broad band.
Physiotherapy cabinets are equipped with pilot setups (such as “Polus”). But the
mechanism of magnetic influence on the whole organism as well as on a separate
cell still remains unexplained that restrains the further development and use of the
methods of magnetotherapy.
The literary data show two possible interaction mechanisms of the magnetic fields
and biological objects: its detection by an organism connected to electromagnetic
induction phenomena, and the interaction of the field with object magnetic material
[293]. Both mechanisms cause different changes in tissues and even their deformations, which can be recorded by coherent-optical methods. As it is shown, electrical
points induced by magnetic field influence, primarily, the excitable structures (nerve
and muscle tissues). In the work [294], it was mentioned the functioning of the first
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