2.2 Holographic Study of Structural and Functional Characteristics …
97
2.2.4 Isolated Preparations—Adequate Experimental Model
of the Study of the Influence of Laser Magnetic Fields,
Hyperbary on the Excitability of Nerve and Muscular
Tissues
Recently, we have observed the increase of scientific interest and importance of
the model studies using isolated preparations. The results of such studies enable
to differentially assess the influence of laser and magnetic fields, hyperbary on the
definite vital activity links.
The object of the study of the influence of these factors on nerve and muscular
tissues were isolated preparations of single nerve fibers, nerve fibers composed of
nerve trunk, isolated single muscle fibers, muscles. The preparation of isolated nerve
was produced by separation of frog sciatic nerve. Preparation method was typical,
the nerve was cut above and below of preliminary ligations, which were used, if
needed, for fixing and transporting of the nerve to special hyperbaric chambers.
Single isolated nerve fibers were prepared from the frog sciatic nerve diameter of
8–10 μm at the length of the prepared area of 5–7 mm). All preparation processes of
single isolated nerve fiber were made according to the method described by I. Tasaki
[292]. Before putting the preparation into hyperbaric chambers, electrophysiological
control of nerve tissue vital activity was made. Nerve fiber excitability, composed of
the isolated nerve trunk, was estimated by its amplitude, action potential time and
the quantity of threshold electrostimulation, which causes the potential generation
in the preparation. For the graph construction of the relations time-force, potential
generation threshold was defined in response to electric stimulation by pulses with
time of 50, 100, 200 and 500 μs.
The stimulation and recording of action potential were made through bipolar
metal electrode hermetically built in hyperbaric chambers. Electrostimulation,
strengthening and recording of action potential were made using electromyography
“Medikor”.
For separation of single muscle fiber, we prepared biceps of frog hip and studied it
under the microscope MBS-9. Muscle fibers were separated by electrolytically edged
steel needles under microscope control. The rest part of the muscle was removed.
Separated fiber was placed in Ringer’s solution for cold-blooded animals.
In the experiment, only intact fibers were used, contracting in response to electrostimulation. Two types of chambers were designed and manufactured for the effect
of increased pressure on the nerve and muscle tissue under study: one to maintain
gas pressure in the range of 0–5 atm., the other to create hydrostatic pressures in the
range of 0–200 atm.
In the works [1–3, 5], the possibility of applying the methods of holographic
interference microscopy for studying the nerve fibers in in vivo state and lymphocytes
was first demonstrated.
These studies allowed obtaining holographic images of the main structural
formations (myelin sheath, Schwann’s cell perikaryon, Ranvie interception structure details). In addition, a significant difference in phase incursions was observed,
97
2.2.4 Isolated Preparations—Adequate Experimental Model
of the Study of the Influence of Laser Magnetic Fields,
Hyperbary on the Excitability of Nerve and Muscular
Tissues
Recently, we have observed the increase of scientific interest and importance of
the model studies using isolated preparations. The results of such studies enable
to differentially assess the influence of laser and magnetic fields, hyperbary on the
definite vital activity links.
The object of the study of the influence of these factors on nerve and muscular
tissues were isolated preparations of single nerve fibers, nerve fibers composed of
nerve trunk, isolated single muscle fibers, muscles. The preparation of isolated nerve
was produced by separation of frog sciatic nerve. Preparation method was typical,
the nerve was cut above and below of preliminary ligations, which were used, if
needed, for fixing and transporting of the nerve to special hyperbaric chambers.
Single isolated nerve fibers were prepared from the frog sciatic nerve diameter of
8–10 μm at the length of the prepared area of 5–7 mm). All preparation processes of
single isolated nerve fiber were made according to the method described by I. Tasaki
[292]. Before putting the preparation into hyperbaric chambers, electrophysiological
control of nerve tissue vital activity was made. Nerve fiber excitability, composed of
the isolated nerve trunk, was estimated by its amplitude, action potential time and
the quantity of threshold electrostimulation, which causes the potential generation
in the preparation. For the graph construction of the relations time-force, potential
generation threshold was defined in response to electric stimulation by pulses with
time of 50, 100, 200 and 500 μs.
The stimulation and recording of action potential were made through bipolar
metal electrode hermetically built in hyperbaric chambers. Electrostimulation,
strengthening and recording of action potential were made using electromyography
“Medikor”.
For separation of single muscle fiber, we prepared biceps of frog hip and studied it
under the microscope MBS-9. Muscle fibers were separated by electrolytically edged
steel needles under microscope control. The rest part of the muscle was removed.
Separated fiber was placed in Ringer’s solution for cold-blooded animals.
In the experiment, only intact fibers were used, contracting in response to electrostimulation. Two types of chambers were designed and manufactured for the effect
of increased pressure on the nerve and muscle tissue under study: one to maintain
gas pressure in the range of 0–5 atm., the other to create hydrostatic pressures in the
range of 0–200 atm.
In the works [1–3, 5], the possibility of applying the methods of holographic
interference microscopy for studying the nerve fibers in in vivo state and lymphocytes
was first demonstrated.
These studies allowed obtaining holographic images of the main structural
formations (myelin sheath, Schwann’s cell perikaryon, Ranvie interception structure details). In addition, a significant difference in phase incursions was observed,