82
L. Acosta-Soto and S. Hosseini
Table 3.1
(continued)
BioMEMS platform
Main components
Fabrication strategy
Mechanisms of
operation
Detected analyte
Specifics
Ref
• Perceval sensor
• PCR
The strategy for
development of
PercevalHR was
based upon mutating
the original Perceval
sensor around the
GlnK
nucleotide-binding
site
Relative
concentrations of ATP
to ADP within cells
was used to monitor
the metabolic activity
of cells. Once the
molecule has
permeated the
cytoplasmic
membrane,
fluorometric response
to different excitation
levels were monitored
to determine the
ATP:ADP ratio
Metabolic activity of
cells
Although it brings a
significant
improvement in
detection range
over the first
iteration, it still falls
short of the total
range that can be
found within cells
Tantama et al.
(2013)
The analyte of interest, and the advantages and disadvantages of each platform are presented in this table
Annexin V (Ann-V); Camptothecin (CAMPT); Computer numerical control (CNC); Direct current (DC); Double-strand DNA (dsDNA); Concentration gradient
generating chip (CGG); Fluorescein isothiocyanate (FITC); Idium tin oxide (ITO); Immuno-globulin G (IgG); Iso-propyl Alcohol (IPA); Lab on chip (LOC);
Laser-Induced Fluorescence (LIF); Microfluidic paper based analytical device (µPAD); Organic electroluminescent diode (OLED); Phosphatidyl-serine (Ps);
Polydimethylsiloxane (PDMS); Polycarbonate (PC); Polymerase chain reaction (PCR); Quantum dots (QD); Terbium (Tb); Type 1 diabetes (T1D); Whatman
filter paper (WFP); Zinc oxide nano-rods (Zn-NRs)
L. Acosta-Soto and S. Hosseini
Table 3.1
(continued)
BioMEMS platform
Main components
Fabrication strategy
Mechanisms of
operation
Detected analyte
Specifics
Ref
• Perceval sensor
• PCR
The strategy for
development of
PercevalHR was
based upon mutating
the original Perceval
sensor around the
GlnK
nucleotide-binding
site
Relative
concentrations of ATP
to ADP within cells
was used to monitor
the metabolic activity
of cells. Once the
molecule has
permeated the
cytoplasmic
membrane,
fluorometric response
to different excitation
levels were monitored
to determine the
ATP:ADP ratio
Metabolic activity of
cells
Although it brings a
significant
improvement in
detection range
over the first
iteration, it still falls
short of the total
range that can be
found within cells
Tantama et al.
(2013)
The analyte of interest, and the advantages and disadvantages of each platform are presented in this table
Annexin V (Ann-V); Camptothecin (CAMPT); Computer numerical control (CNC); Direct current (DC); Double-strand DNA (dsDNA); Concentration gradient
generating chip (CGG); Fluorescein isothiocyanate (FITC); Idium tin oxide (ITO); Immuno-globulin G (IgG); Iso-propyl Alcohol (IPA); Lab on chip (LOC);
Laser-Induced Fluorescence (LIF); Microfluidic paper based analytical device (µPAD); Organic electroluminescent diode (OLED); Phosphatidyl-serine (Ps);
Polydimethylsiloxane (PDMS); Polycarbonate (PC); Polymerase chain reaction (PCR); Quantum dots (QD); Terbium (Tb); Type 1 diabetes (T1D); Whatman
filter paper (WFP); Zinc oxide nano-rods (Zn-NRs)