3 Bio-microelectromechanical Systems (BioMEMS) in Bio-sensing …
81
Table 3.1
(continued)
BioMEMS platform
Main components
Fabrication strategy
Mechanisms of
operation
Detected analyte
Specifics
Ref
• Functionalized
QDs
• Organic quencher
(BHQ2)
The QDs were
conjugated to DNA
strands designed to
bind to analyte of
interest
The sample was
prepared by exposure
to the analyte marker.
In the presence of the
analyte is present, will
interact with the QD
and quench the
fluorescent signal.
The mechanism of
operation was based
on Förster resonance
energy transfer
(FRET)
• DNA
• MicroRNA
The DNA-QDs is
benefited with
excellent optical
properties of QDs
while using tailored
properties of DNA
for detection hence
act as nano-sensors
for nucleic acid
detection
Su
et al.(2014)
• AuNC
• FITC
• PBS
AuNC was exposed to
PBS to form
PBA-AuNC. FITC
was conjugated to this
coupling complex and
FA was subsequently
incorporated to
improve cellular
membrane
permeability
The complex of
FA-FITC-BSA-AuNC
demonstrates a high
affinity towards the
folate acceptor
proteins on the
cytoplasmic
membranes of Hela
cells. Once it has
crossed the
membrane, it can be
used to monitor
changes in pH as a
function of the ratio of
the stable AuNC
emission in contract
to that of FITC
Intracellular pH
The AuNC is
highly permeable to
the cytoplasmic
membrane of
cancerous cells
Ding and Tian
(2014)
(continued)