3 Bio-microelectromechanical Systems (BioMEMS) in Bio-sensing …
71
Table 3.1
Recent BioMEMS platforms for fluorescent detection: Type of the platform, main components, fabrication strategy, mechanism of operation
BioMEMS platform
Main components
Fabrication strategy
Mechanisms of
operation
Detected analyte
Specifics
Ref
µPAD
• ZnO-NRs
• WFP
The paper device was
fabricated by treating
WFP with a standard
hydrothermal process
to grow ZnO-NRs.
Using salinization
chemistry, capture
antibodies were
bound to the surface
of the treated paper
Functionalized paper
was exposed to
sample flow to
promote
analyte-surface
interaction followed
by exposure to HRP
conjugated secondary
antibodies for the final
readout
Cardiac Myoglobin
The
µPAD can be
used both as a
passive
pre-concentrator
and an analytical
device itself
Tiwari et al.
(2017)
• WFP
• CBM-ZZ
• IgG-FITC
Micro-channels are
wax printed onto the
surface of the WFP
and then melted. WFP
was exposed to
CBM-ZZ. IgG-FITC
was deposited on to
the paper for
subsequent detection
The analyte sample
was directed in the
paper by capillary
forces through
different analysis
stages for
biorecognition
Biotinylated-DNA
The final test
performed showed
little to no detection
of analyte despite
its known presence,
leaving room for
improvement of the
device
Rosa et al.
(2014)
(continued)
71
Table 3.1
Recent BioMEMS platforms for fluorescent detection: Type of the platform, main components, fabrication strategy, mechanism of operation
BioMEMS platform
Main components
Fabrication strategy
Mechanisms of
operation
Detected analyte
Specifics
Ref
µPAD
• ZnO-NRs
• WFP
The paper device was
fabricated by treating
WFP with a standard
hydrothermal process
to grow ZnO-NRs.
Using salinization
chemistry, capture
antibodies were
bound to the surface
of the treated paper
Functionalized paper
was exposed to
sample flow to
promote
analyte-surface
interaction followed
by exposure to HRP
conjugated secondary
antibodies for the final
readout
Cardiac Myoglobin
The
µPAD can be
used both as a
passive
pre-concentrator
and an analytical
device itself
Tiwari et al.
(2017)
• WFP
• CBM-ZZ
• IgG-FITC
Micro-channels are
wax printed onto the
surface of the WFP
and then melted. WFP
was exposed to
CBM-ZZ. IgG-FITC
was deposited on to
the paper for
subsequent detection
The analyte sample
was directed in the
paper by capillary
forces through
different analysis
stages for
biorecognition
Biotinylated-DNA
The final test
performed showed
little to no detection
of analyte despite
its known presence,
leaving room for
improvement of the
device
Rosa et al.
(2014)
(continued)