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M. A. Espinosa-Hernandez et al.
Fig. 2.22 a Schematic of centrifugal LAMP microdevice, btop view of one unit of the microdevice,
c photograph of the microdevice, d schematic top view of the device’s layers (Sayad et al. 2017)
channel between the metering and amplification chambers to prohibit liquid evaporation. Lastly, the amplification chamber was designed for the amplification and
detection of DNA. An optimized square-wave microchannel, metering chambers,
and revulsion per minute (RPM) control were utilized to constantly load, mix, and
aliquot the LAMP primers/reagents as well as DNA samples. The LAMP reaction
amplicons were detected by the calcein dye colorimetric method and analyzed with
the developed electronic endpoint detection system (Fig. 2.23a) including the Bluetooth interface to send the results to a smartphone (Fig. 2.23b). Calcein is a synthetic
fluorescein that emits a bright fluorescence creating a visual color change. A positive
sample changes from yellow to green while a negative readout remains light orange.
The entire process in only one CD lasted about 65 min and presented a LOD of 3 ×
10
–5 ng μL
−1 .
M. A. Espinosa-Hernandez et al.
Fig. 2.22 a Schematic of centrifugal LAMP microdevice, btop view of one unit of the microdevice,
c photograph of the microdevice, d schematic top view of the device’s layers (Sayad et al. 2017)
channel between the metering and amplification chambers to prohibit liquid evaporation. Lastly, the amplification chamber was designed for the amplification and
detection of DNA. An optimized square-wave microchannel, metering chambers,
and revulsion per minute (RPM) control were utilized to constantly load, mix, and
aliquot the LAMP primers/reagents as well as DNA samples. The LAMP reaction
amplicons were detected by the calcein dye colorimetric method and analyzed with
the developed electronic endpoint detection system (Fig. 2.23a) including the Bluetooth interface to send the results to a smartphone (Fig. 2.23b). Calcein is a synthetic
fluorescein that emits a bright fluorescence creating a visual color change. A positive
sample changes from yellow to green while a negative readout remains light orange.
The entire process in only one CD lasted about 65 min and presented a LOD of 3 ×
10
–5 ng μL
−1 .