2 Bio-microelectromechanical Systems (BioMEMS) …
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with a DVD diagnostic software and the readings were interpreted according to the
radial distance and optical darkness ratio. The results showed comparable sensitivity
and selectivity to well-established colorimetric methods and ELISA (Fig. 2.19b).
Additionally, it is an inexpensive, easy to use, multiplex, POC diagnostic instrument
for prompt response used in remote and/or rural areas.
Park et al. (2016) developed an integrated rotary microfluidic system (Fig. 2.20a)
with DNA extraction unit [Fig. 2.20c(I)], LAMP [Fig. 2.20c(II)], and lateral
flow strip [Fig. 2.20c(III)]. The device was used for detection of the food-borne
bacterial pathogen, monoplex Salmonella Typhimurium and multiplex Salmonella
Typhimurium as well as Vibrio parahaemolyticus. The LAMP reaction replaced
Fig. 2.20 a Image of integrated rotary microdevice, b illustration of integrated rotary microdevice, c(I) schematic of solid phase DNA extraction and fluorescent images of the adsorbed glass
microbeads, c(II) schematic of LAMP amplification of target DNA, c(III) schematic of lateral flow
strip detection (Park et al. 2016)
59
with a DVD diagnostic software and the readings were interpreted according to the
radial distance and optical darkness ratio. The results showed comparable sensitivity
and selectivity to well-established colorimetric methods and ELISA (Fig. 2.19b).
Additionally, it is an inexpensive, easy to use, multiplex, POC diagnostic instrument
for prompt response used in remote and/or rural areas.
Park et al. (2016) developed an integrated rotary microfluidic system (Fig. 2.20a)
with DNA extraction unit [Fig. 2.20c(I)], LAMP [Fig. 2.20c(II)], and lateral
flow strip [Fig. 2.20c(III)]. The device was used for detection of the food-borne
bacterial pathogen, monoplex Salmonella Typhimurium and multiplex Salmonella
Typhimurium as well as Vibrio parahaemolyticus. The LAMP reaction replaced
Fig. 2.20 a Image of integrated rotary microdevice, b illustration of integrated rotary microdevice, c(I) schematic of solid phase DNA extraction and fluorescent images of the adsorbed glass
microbeads, c(II) schematic of LAMP amplification of target DNA, c(III) schematic of lateral flow
strip detection (Park et al. 2016)