2 Bio-microelectromechanical Systems (BioMEMS) …
33
Table 2.1
(continued)
BioMEMS platform
Main components
Fabrication strategy Mechanisms of
operation
Detected analyte
Specifics
References
Molecular diagnostic
quantitative pregnancy
test on a DVD
• DVD
• Computer optical
drive
The DVD’s PS
surface underwent
activation by UV
irradiation and
treatment with EDC
and NHS. A PDMS
plate was added with
microchannels.
Anti-hCGα were
immobilized on the
surface, streptavidin
nanogold conjugates
were bound and a
silver staining
treatment was
performed
Samples were loaded
into the PDMS
microfluidic channels
on the DVD. The DVD
was spun, and the
samples create a
different radial
distance depending on
the concentration of
the analyte. The assay
was tested with a
diagnostic software
and evaluated based on
radial distance and
optical darkness ratio
• hCG
Th strategy
provided a low-cost
POC tool for
worldwide testing
and comparable
results to common
immunoassays and
ELISA
Li et al.
(2014)
(continued)