2 Bio-microelectromechanical Systems (BioMEMS) …
27
Table 2.1
(continued)
BioMEMS platform
Main components
Fabrication strategy Mechanisms of
operation
Detected analyte
Specifics
References
Paper/polymer hybrid
μPAD microplate
• PMMA
• Chromatography
paper disks
• Microwells
Micro-machining
using laser ablation
was used. Pieces of
chromatography
paper were cut using
a laser cutter and
placed inside the
device. To assemble
the device, different
PMMA layers were
clamped together
Assay reagents were
loaded in the top
PMMA layer. Then,
the reagents arrived to
the chromatography
paper in each
microwell by capillary
forces and allowed the
excess of it flow to the
third layer for removal
• HBsAg
• IgG
The use of
paper-based
technology resulted
in rapid
immobilization of
antibody/ antigen
and avoided
complicated
surface
modifications. The
device was simple,
portable, rapid and
highly sensitive
Sanjay et al.
(2016)
Double-layered
μPAD • Nylon mesh
• Whatman paper
The 3D microfluidic
channels were made
through traditional
wax-screen printing
technology
A blood sample was
dispensed on sampling
zone, and passed
through the
hydrophobic channels
in order to react with
the reactants. The
color intensity was
recorded by an Image J
software
• Glucose
• Uric acid
• Lactate
• Choline
This device allowed
the detection of
four biomolecules
simultaneously, and
improved
colorimetric
performance,
sensitivity, and
detection range
Li et al.
(2018)
(continued)
27
Table 2.1
(continued)
BioMEMS platform
Main components
Fabrication strategy Mechanisms of
operation
Detected analyte
Specifics
References
Paper/polymer hybrid
μPAD microplate
• PMMA
• Chromatography
paper disks
• Microwells
Micro-machining
using laser ablation
was used. Pieces of
chromatography
paper were cut using
a laser cutter and
placed inside the
device. To assemble
the device, different
PMMA layers were
clamped together
Assay reagents were
loaded in the top
PMMA layer. Then,
the reagents arrived to
the chromatography
paper in each
microwell by capillary
forces and allowed the
excess of it flow to the
third layer for removal
• HBsAg
• IgG
The use of
paper-based
technology resulted
in rapid
immobilization of
antibody/ antigen
and avoided
complicated
surface
modifications. The
device was simple,
portable, rapid and
highly sensitive
Sanjay et al.
(2016)
Double-layered
μPAD • Nylon mesh
• Whatman paper
The 3D microfluidic
channels were made
through traditional
wax-screen printing
technology
A blood sample was
dispensed on sampling
zone, and passed
through the
hydrophobic channels
in order to react with
the reactants. The
color intensity was
recorded by an Image J
software
• Glucose
• Uric acid
• Lactate
• Choline
This device allowed
the detection of
four biomolecules
simultaneously, and
improved
colorimetric
performance,
sensitivity, and
detection range
Li et al.
(2018)
(continued)