8 Bio-microelectromechanical Systems (BioMEMS) …
167
Table 8.1
(continued)
BioMEMS platform
Main components
Fabrication strategy
Mechanisms of operation Detected analyte
Specifics
References
Cell-on-a-Chip (COC)
• Microdisc Electrode
Array
• Ag/AgCl reference
electrode
• Platinum mesh counter
electrode
The device was fabricated
by lithography technique
and the electrochemical
transducers were
fabricated from electron
beam vapor deposited
platinum on an adhesion
promoting Ti/W layer
onto thick electronics
grade borosilicate glass
A competitive ELISA was
conducted using OTA and
OTA-HRP solutions. The
devices were dipped into
the luminol-hydrogen
peroxide solution and the
amperometric response
was recorded continually
by the potentiostat and
periodically by the
luminometer
Ochratoxin A
The device presented a
simultaneous
amperometric and
chemiluminescence
recordings
Tria et al. 2016)
Electrochemical
immunosensor
• Silanized silicon mold
platform
• PDMS coating,
polyclonal antibodies
for antigen measurement
• MNPs
•
µWEs
The electrodes were
modified by
electro-addressing
diazonium salt and
electrodepositing
magnetic nanoparticles
coated with
(Py/Py-COOH/MNPs).
The Py/Py-COOH/MNPs
coated core-shell magnetic
nanoparticles were
synthesized through
utilization of a
seeded-polymerization
technique
TC immobilization on the
µWEs surface was
performed by
functionalization with
CMA. Activated
electrode surfaces were
incubated with TC. Via a
second functionalization
method, CMA was used
for the activation of the
µWEs followed by
incubation with MNPs
coated
Py/Py-COOH/MNPs
cross-linked with Ab-TC
Tetracycline
The immunosensor was
capable of dramatically
decreasing the time of
analysis
Wang et al. (2018)
(continued)
167
Table 8.1
(continued)
BioMEMS platform
Main components
Fabrication strategy
Mechanisms of operation Detected analyte
Specifics
References
Cell-on-a-Chip (COC)
• Microdisc Electrode
Array
• Ag/AgCl reference
electrode
• Platinum mesh counter
electrode
The device was fabricated
by lithography technique
and the electrochemical
transducers were
fabricated from electron
beam vapor deposited
platinum on an adhesion
promoting Ti/W layer
onto thick electronics
grade borosilicate glass
A competitive ELISA was
conducted using OTA and
OTA-HRP solutions. The
devices were dipped into
the luminol-hydrogen
peroxide solution and the
amperometric response
was recorded continually
by the potentiostat and
periodically by the
luminometer
Ochratoxin A
The device presented a
simultaneous
amperometric and
chemiluminescence
recordings
Tria et al. 2016)
Electrochemical
immunosensor
• Silanized silicon mold
platform
• PDMS coating,
polyclonal antibodies
for antigen measurement
• MNPs
•
µWEs
The electrodes were
modified by
electro-addressing
diazonium salt and
electrodepositing
magnetic nanoparticles
coated with
(Py/Py-COOH/MNPs).
The Py/Py-COOH/MNPs
coated core-shell magnetic
nanoparticles were
synthesized through
utilization of a
seeded-polymerization
technique
TC immobilization on the
µWEs surface was
performed by
functionalization with
CMA. Activated
electrode surfaces were
incubated with TC. Via a
second functionalization
method, CMA was used
for the activation of the
µWEs followed by
incubation with MNPs
coated
Py/Py-COOH/MNPs
cross-linked with Ab-TC
Tetracycline
The immunosensor was
capable of dramatically
decreasing the time of
analysis
Wang et al. (2018)
(continued)