6 Bio-microelectromechanical Systems (BioMEMS) …
145
Fig. 6.6 a A schematic illustration of the experimental process for measuring Hb and HbA1c. b A
schematic illustration of the experimental procedure performed on the integrated microfluidic chip
(Chang et al. 2015)
target-aptamer-bead complexes in a 10-min incubation, acridinium ester-labeled Hb
or HbA1c specific antibodies were injected into the transportation unit. The washing
process was performed for the elimination of unbound antibodies and the magnetic
collection. Finally, to re-suspend the sandwich-like complex of magnetic beads,
the CL reagents were pumped into the closed chamber. Concurrently, a portable
luminescence detection was implemented to measure the chemiluminescent signals
while quantifying the concentrations of Hb or HbA1c. The compact microfluidic
system consumed less samples and reagents and significantly shortened the detection
time. The authors eliminated the use of primary antibody, which decreased the cost
of the assay. The presented device has the potential to be used for diabetes screening
145
Fig. 6.6 a A schematic illustration of the experimental process for measuring Hb and HbA1c. b A
schematic illustration of the experimental procedure performed on the integrated microfluidic chip
(Chang et al. 2015)
target-aptamer-bead complexes in a 10-min incubation, acridinium ester-labeled Hb
or HbA1c specific antibodies were injected into the transportation unit. The washing
process was performed for the elimination of unbound antibodies and the magnetic
collection. Finally, to re-suspend the sandwich-like complex of magnetic beads,
the CL reagents were pumped into the closed chamber. Concurrently, a portable
luminescence detection was implemented to measure the chemiluminescent signals
while quantifying the concentrations of Hb or HbA1c. The compact microfluidic
system consumed less samples and reagents and significantly shortened the detection
time. The authors eliminated the use of primary antibody, which decreased the cost
of the assay. The presented device has the potential to be used for diabetes screening