6 Bio-microelectromechanical Systems (BioMEMS) …
133
Table 6.1
(continued)
BioMEMS platform
Main components
Fabrication strategy
Mechanisms of operation Detected analyte
Specifics
References
Tri-layer microfluidic
chip
• Magnetic beads
• Base-paired DNA
aptamers labeled with
biotins
• PDMS
• Glass
A CNC machined the
PMMA as the master
mold with the inverse
microstructures. PDMS
was used via replicate
molding process. This
process was done to create
both thin and thick layers.
Finally, the two layers and
the glass substrate were
bonded using oxygen
plasma treatment
The magnetic beads were
coated with the aptamers,
transported to the central
chamber of the device,
and incubated with the
blood samples. The target
antibody was added and
the sandwich structure
was then collected by a
magnetic field to wash
non-binding agents.
Finally, the
target-aptamer-bead
complex was tranferred to
the NaOH chamber where
the CL signal was
detected
HbA1c
Automated detection
during 25 min on a single
chip was effectively
demonstrated
Chang et al. (2015)
Smartphone-based
biosensor
• LG G4 smartphone
• Dark PMMA box
The dark box was
constructed using black
PMMA sheets, then
joined using epoxy glue
To perform CL reaction,
potassium permanganate
reagent was added on the
analyte on the TLC plate,
and the emitted light was
captured by a smartphone
camera
Morphine
The method was simple
and cost-effective
Shahvar et al. (2018)
Fiber-optic biosensor
Optical fiber
Optic fibers were cleaned
and treated with piranha
solution to produce
hydroxyl groups
The target molecules were
immobilized on the
hydroxyl groups of the
fibers to bind to
complementary labeled
detection antibodies and
to record the signal
CCHF virus IgG
antibodies
The protocol showed a
100-fold greater
sensitivity for detection
of CCHF antibodies
Algaar et al. (2015)
(continued)
133
Table 6.1
(continued)
BioMEMS platform
Main components
Fabrication strategy
Mechanisms of operation Detected analyte
Specifics
References
Tri-layer microfluidic
chip
• Magnetic beads
• Base-paired DNA
aptamers labeled with
biotins
• PDMS
• Glass
A CNC machined the
PMMA as the master
mold with the inverse
microstructures. PDMS
was used via replicate
molding process. This
process was done to create
both thin and thick layers.
Finally, the two layers and
the glass substrate were
bonded using oxygen
plasma treatment
The magnetic beads were
coated with the aptamers,
transported to the central
chamber of the device,
and incubated with the
blood samples. The target
antibody was added and
the sandwich structure
was then collected by a
magnetic field to wash
non-binding agents.
Finally, the
target-aptamer-bead
complex was tranferred to
the NaOH chamber where
the CL signal was
detected
HbA1c
Automated detection
during 25 min on a single
chip was effectively
demonstrated
Chang et al. (2015)
Smartphone-based
biosensor
• LG G4 smartphone
• Dark PMMA box
The dark box was
constructed using black
PMMA sheets, then
joined using epoxy glue
To perform CL reaction,
potassium permanganate
reagent was added on the
analyte on the TLC plate,
and the emitted light was
captured by a smartphone
camera
Morphine
The method was simple
and cost-effective
Shahvar et al. (2018)
Fiber-optic biosensor
Optical fiber
Optic fibers were cleaned
and treated with piranha
solution to produce
hydroxyl groups
The target molecules were
immobilized on the
hydroxyl groups of the
fibers to bind to
complementary labeled
detection antibodies and
to record the signal
CCHF virus IgG
antibodies
The protocol showed a
100-fold greater
sensitivity for detection
of CCHF antibodies
Algaar et al. (2015)
(continued)