126
A. S. Cerda-Kipper and S. Hosseini
Table 6.1
(continued)
BioMEMS platform
Main components
Fabrication strategy
Mechanisms of operation Detected analyte
Specifics
References
CL-LFIA biosensor
• LFIA strip
• Ultrasensitive cooled
CCD camera
• 3D printed cartridge
• OFSE
Assay strips for LFIA
were prepared by
immobilizing, the rabbit
anti-cortisol antibody
(T-line) and the rabbit
anti-HRP antibody
(C-line), at different
dilutions onto
nitrocellulose membranes,
which were saturated with
BSA and washed with
PBS including Tween 20.
The membranes were
subsequently assembled
with adsorbent pads and
sample, then cut into
small segments
The oral sample was
loaded via pushing the
syringe plunger for filling
the sample metering
chamber. One of the 3
valves were opened and
the HRP-cortisol
conjugate and sample
were transferred to the
LFIA strip. The reaction
mixture was sent to HRP
CL substrate after
incubation, and the CL
signal acquisition was
performed
Cortisol
The manifestation of the
suitability of quantitative
CL-LFIA for space
applications set the stage
for prospective
developments of
biosensing systems for
multiple and early
diagnosis of different
analytes in space
Zangheri et al. (2019)
(continued)