Chapter 6
Bio-microelectromechanical Systems
(BioMEMS) in Bio-sensing
Applications-Chemiluminescence
Detection Strategies
Ana Sofia Cerda-Kipper and Samira Hosseini
6.1 Introduction
An attractive detection method for the development of biosensors is chemiluminescence (CL), that identifies the presence and quantifies the concentration of an analyte
by using luminescent agents that change their optical properties as a result of a chemical reaction. Over time, application of CL has become more frequent in detection of
proteins and oligonucleotides in immunoassay and DNA probe assays, such as DNA
sequencing and detection (Smith et al. 2018). Compared to other detection strategies
including calorimetric, and fluorescence, CL offers great beneficial features thanks to
its low background signal, simple readout methods, and inherent sensitivity (Xu et al.
2010). When combined with bio-microelectromechanical systems (BioMEMS), CL
can offer a powerful strategy for timely and sensitive detection of various analytes in
remote and rural areas and for extreme point of care (EPOC) (Wojciechowski et al.
2009).
6.2 Chemiluminescence Detection Strategy
The emission of light due to certain chemical reactions is known as CL. This emission
which is commonly produced in the visible or near infrared spectral regions, is
the result of an excited electronic molecular state, formed in a chemical reaction,
returning to the ground state of energy through non-radiative processes (Zagatto
et al. 2012; Poole 2003). Coupling of CL with other detection strategies such as
fluorescent or colorimetric could offer higher sensitivity and multiply the detectable
products, which, in turn, can be read via different means (Bridle 2014). In this chapter
A. S. Cerda-Kipper · S. Hosseini (B)
School of Engineering and Sciences, Tecnologico de Monterrey, Monterrey, Mexico
e-mail: samira.hosseini@tec.mx
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer
Nature Singapore Pte Ltd. 2021
S. Hosseini et al., BioMEMS, Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-6382-9_6
123
Bio-microelectromechanical Systems
(BioMEMS) in Bio-sensing
Applications-Chemiluminescence
Detection Strategies
Ana Sofia Cerda-Kipper and Samira Hosseini
6.1 Introduction
An attractive detection method for the development of biosensors is chemiluminescence (CL), that identifies the presence and quantifies the concentration of an analyte
by using luminescent agents that change their optical properties as a result of a chemical reaction. Over time, application of CL has become more frequent in detection of
proteins and oligonucleotides in immunoassay and DNA probe assays, such as DNA
sequencing and detection (Smith et al. 2018). Compared to other detection strategies
including calorimetric, and fluorescence, CL offers great beneficial features thanks to
its low background signal, simple readout methods, and inherent sensitivity (Xu et al.
2010). When combined with bio-microelectromechanical systems (BioMEMS), CL
can offer a powerful strategy for timely and sensitive detection of various analytes in
remote and rural areas and for extreme point of care (EPOC) (Wojciechowski et al.
2009).
6.2 Chemiluminescence Detection Strategy
The emission of light due to certain chemical reactions is known as CL. This emission
which is commonly produced in the visible or near infrared spectral regions, is
the result of an excited electronic molecular state, formed in a chemical reaction,
returning to the ground state of energy through non-radiative processes (Zagatto
et al. 2012; Poole 2003). Coupling of CL with other detection strategies such as
fluorescent or colorimetric could offer higher sensitivity and multiply the detectable
products, which, in turn, can be read via different means (Bridle 2014). In this chapter
A. S. Cerda-Kipper · S. Hosseini (B)
School of Engineering and Sciences, Tecnologico de Monterrey, Monterrey, Mexico
e-mail: samira.hosseini@tec.mx
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer
Nature Singapore Pte Ltd. 2021
S. Hosseini et al., BioMEMS, Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-6382-9_6
123