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A. S. Cerda-Kipper and S. Hosseini
sensitivity to substitute transportable light detectors including CCD and photodiodies
for measurement of the analyte’s medium–high concentrations. The smartphoneintegrated CMOS were tested as optical biosensors for numerous biosensing
methods based on fluorescence, electrochemiluminescence, and biochemiluminescence (Cevenini et al. 2016a).
Cevenini et al. (2016b) previously presented the integration of genetically engineered cells within a 3D-printed smartphone device as a low-cost and user-friendly
toxicity level using bioluminescent sentinel cells (Cevenini et al. 2016b). Later, the
authors reported the advancement of a BL smartphone-based cell biosensor utilizing
NanoLuc luciferase reporter for measureable evaluation of anti-inflammatory activity
and toxicity of grape extracts. This study used purified fusion proteins including
NanoLuc as a BL donor as well as mNeonGreen fluorescent protein that acted as
a receptor. The coupling of the target antibody with the analyte resulted in a color
change from green–blue to blue which was received by a smartphone camera and
was subsequently processed by a custom-developed app. This platform presented
a reliable tool for pre-screening and sample selection for customized and effective
analysis (Cevenini et al. 2016a).
5.5 Summary
Bioluminescence (BL) maintains the emission as it is a natural property in some
of the living organisms and therefore offers excellent sensitivity and suitability for
noninvasive in-vivo imaging. Bioluminescence represents an exceptional mechanism
for the development of ultrasensitive analytical and bioanalytical protocols. The
BioMEMS that operate based upon the principles of BL are commonly benefited
from sensitivity, analytical speed, non-hazardous reagents, and simple procedures
in a range of applications including immunoassay, protein blotting, and DNA probe
assays.
References
Caputo D et al (2017) Integrated system based on thin film technologies for cell-based bioluminescence assays. In: Proceedings, vol. 1, no. 10, p. 513, Aug. 2017. https://doi.org/10.3390/procee
dings1040513
Cevenini L et al (2016a) Exploiting NanoLuc luciferase for smartphone-based bioluminescence cell
biosensor for (anti)-inflammatory activity and toxicity. Anal Bioanal Chem 408(30):8859–8868.
https://doi.org/10.1007/s00216-016-0062-3
Cevenini L, Calabretta MM, Tarantino G, Michelini E, Roda A (2016b) Smartphone-interfaced 3D
printed toxicity biosensor integrating bioluminescent ‘sentinel cells.’ Sensors Actuators B Chem
225:249–257. https://doi.org/10.1016/JSNB.2015.11.017
Comina G, Suska A, Filippini D (2014) Low cost lab-on-a-chip prototyping with a consumer grade
3D printer. Lab Chip 14(16):2978–2982. https://doi.org/10.1039/c4lc00394b
A. S. Cerda-Kipper and S. Hosseini
sensitivity to substitute transportable light detectors including CCD and photodiodies
for measurement of the analyte’s medium–high concentrations. The smartphoneintegrated CMOS were tested as optical biosensors for numerous biosensing
methods based on fluorescence, electrochemiluminescence, and biochemiluminescence (Cevenini et al. 2016a).
Cevenini et al. (2016b) previously presented the integration of genetically engineered cells within a 3D-printed smartphone device as a low-cost and user-friendly
toxicity level using bioluminescent sentinel cells (Cevenini et al. 2016b). Later, the
authors reported the advancement of a BL smartphone-based cell biosensor utilizing
NanoLuc luciferase reporter for measureable evaluation of anti-inflammatory activity
and toxicity of grape extracts. This study used purified fusion proteins including
NanoLuc as a BL donor as well as mNeonGreen fluorescent protein that acted as
a receptor. The coupling of the target antibody with the analyte resulted in a color
change from green–blue to blue which was received by a smartphone camera and
was subsequently processed by a custom-developed app. This platform presented
a reliable tool for pre-screening and sample selection for customized and effective
analysis (Cevenini et al. 2016a).
5.5 Summary
Bioluminescence (BL) maintains the emission as it is a natural property in some
of the living organisms and therefore offers excellent sensitivity and suitability for
noninvasive in-vivo imaging. Bioluminescence represents an exceptional mechanism
for the development of ultrasensitive analytical and bioanalytical protocols. The
BioMEMS that operate based upon the principles of BL are commonly benefited
from sensitivity, analytical speed, non-hazardous reagents, and simple procedures
in a range of applications including immunoassay, protein blotting, and DNA probe
assays.
References
Caputo D et al (2017) Integrated system based on thin film technologies for cell-based bioluminescence assays. In: Proceedings, vol. 1, no. 10, p. 513, Aug. 2017. https://doi.org/10.3390/procee
dings1040513
Cevenini L et al (2016a) Exploiting NanoLuc luciferase for smartphone-based bioluminescence cell
biosensor for (anti)-inflammatory activity and toxicity. Anal Bioanal Chem 408(30):8859–8868.
https://doi.org/10.1007/s00216-016-0062-3
Cevenini L, Calabretta MM, Tarantino G, Michelini E, Roda A (2016b) Smartphone-interfaced 3D
printed toxicity biosensor integrating bioluminescent ‘sentinel cells.’ Sensors Actuators B Chem
225:249–257. https://doi.org/10.1016/JSNB.2015.11.017
Comina G, Suska A, Filippini D (2014) Low cost lab-on-a-chip prototyping with a consumer grade
3D printer. Lab Chip 14(16):2978–2982. https://doi.org/10.1039/c4lc00394b