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A. S. Cerda-Kipper and S. Hosseini
respect to the O 2 sensing dye and polymer matrix, 2,3,7,8,12,13,17,18-octaethyl21H,23H-porphyrin, platinum (II) (PtOEP) and polystyrene (PS), were selected,
respectively. Titanium dioxide nanoparticles (TiO 2 NPs) were integrated in PS as a
light scattering center. For the working mechanism, the sensing film was placed in
a close contact with the skin in order to detect tcpO 2 while, around the periphery of
the sensor, a carbon tape was attached to the skin to optically isolate the measurement. The O 2 molecules quenched the phosphorescence in the film that resulted in
the reduction of the PL intensity generated by the sensing film. The bandage-form
sensor detected the dissolved O 2 which upon diffusion from the vessels to the skin
(Fig. 4.2). Furthermore, the wearable O 2 sensor could screen the tcpO 2 in different
parts of the body during exercise or work. To confirm the device performance, the
authors recorded the tcpO 2 variants in the lower arm and a thumb by pressure-induced
occlusion in the wearable sensor. The obtained data were in agreement with those
measured commercially. The main beneficial features of this device were flexibility
and cost-effectiveness that have rendered the sensor as a great candidate for real-time
tcpO 2 monitoring Lim et al. 2018).
4.5 Summary
Luminescent sensing schemes are especially helpful for the recognition of small
molecules and for assesing the involved parameters, and are roughly 100–1000 fold
more sensitive than widely-used colorimetric strategies. The integration of these
sensors into microfluidic systems allows information on microenvironments, which
are very promising, and fast growing, especially in cell culture and organ-on-achip applications. In this chapter microfluidic devices such as lab-on-chip devices
(LOC), and alternative technologies in the field of BioMEMS were reviewed. The
advances in this area, opens various windows of opportunity to future developments
of luminescent-based biosensors.
A. S. Cerda-Kipper and S. Hosseini
respect to the O 2 sensing dye and polymer matrix, 2,3,7,8,12,13,17,18-octaethyl21H,23H-porphyrin, platinum (II) (PtOEP) and polystyrene (PS), were selected,
respectively. Titanium dioxide nanoparticles (TiO 2 NPs) were integrated in PS as a
light scattering center. For the working mechanism, the sensing film was placed in
a close contact with the skin in order to detect tcpO 2 while, around the periphery of
the sensor, a carbon tape was attached to the skin to optically isolate the measurement. The O 2 molecules quenched the phosphorescence in the film that resulted in
the reduction of the PL intensity generated by the sensing film. The bandage-form
sensor detected the dissolved O 2 which upon diffusion from the vessels to the skin
(Fig. 4.2). Furthermore, the wearable O 2 sensor could screen the tcpO 2 in different
parts of the body during exercise or work. To confirm the device performance, the
authors recorded the tcpO 2 variants in the lower arm and a thumb by pressure-induced
occlusion in the wearable sensor. The obtained data were in agreement with those
measured commercially. The main beneficial features of this device were flexibility
and cost-effectiveness that have rendered the sensor as a great candidate for real-time
tcpO 2 monitoring Lim et al. 2018).
4.5 Summary
Luminescent sensing schemes are especially helpful for the recognition of small
molecules and for assesing the involved parameters, and are roughly 100–1000 fold
more sensitive than widely-used colorimetric strategies. The integration of these
sensors into microfluidic systems allows information on microenvironments, which
are very promising, and fast growing, especially in cell culture and organ-on-achip applications. In this chapter microfluidic devices such as lab-on-chip devices
(LOC), and alternative technologies in the field of BioMEMS were reviewed. The
advances in this area, opens various windows of opportunity to future developments
of luminescent-based biosensors.