3 Bio-microelectromechanical Systems (BioMEMS) in Bio-sensing …
93
Fig. 3.9 Build of the multiple phytoplankton biosensor (Shin et al. 2018)
The identification of specific strands of genetic material including DNA or microRNA (miRNA) is of clinical importance to the detection and diagnostic of several
pathologies. A new strategy for detection of such analytes with a high rate of
specificity and selectivity was reported by Su et al. (2014). The method involved
3-mercaptopropionic acid (MPA)-capped QDs functionalized with thiolated DNA
through ligand-exchange. The two reactants were mixed for several hours at different
temperature ranges to achieve complete exchange between the two, resulting in DNAQD conjugates. Förster resonance energy transfer (FRET) mechanism was used to
ensure efficient energy transfer between the QD’s and the BHQ 2 -DNA (Fig. 3.10).
Fig. 3.10 Graphic visualization of FRET mechanism (Su et al. 2014)
93
Fig. 3.9 Build of the multiple phytoplankton biosensor (Shin et al. 2018)
The identification of specific strands of genetic material including DNA or microRNA (miRNA) is of clinical importance to the detection and diagnostic of several
pathologies. A new strategy for detection of such analytes with a high rate of
specificity and selectivity was reported by Su et al. (2014). The method involved
3-mercaptopropionic acid (MPA)-capped QDs functionalized with thiolated DNA
through ligand-exchange. The two reactants were mixed for several hours at different
temperature ranges to achieve complete exchange between the two, resulting in DNAQD conjugates. Förster resonance energy transfer (FRET) mechanism was used to
ensure efficient energy transfer between the QD’s and the BHQ 2 -DNA (Fig. 3.10).
Fig. 3.10 Graphic visualization of FRET mechanism (Su et al. 2014)