250
T. Amemiya et al.
15.6 Glycolytic Oscillations in Prostate Cancer Cells
(DU145)
Prostate cancer, DU145 cells were also found to exhibit glycolytic oscillations for
the first time as shown in Fig. 15.3. Experimental methods for the oscillations in
DU145 cells were similar to those for HeLa cells [5]. We found that the periods of
oscillations (46.2 ± 14.6 s) were longer and the maximum of oscillatory ratio (0.249)
was smaller in DU145 cells than the periods (19.3 ± 6.30 s) and the oscillatory ratio
(0.355) in HeLa cells. DU145 cells also exhibited heterogeneities in the oscillations
in terms of the number of oscillatory cells, periods of oscillations, and duration of
oscillations, similar to HeLa cells [4, 5].
Interestingly, the Warburg effect, a common feature of cancer cells, is not observed
in prostate cancer cells [18]. In particular, early prostate cancers rely on lipids
and other energetic molecules, and not on aerobic respiration, for ATP production.
Fig. 15.3 Typical time series of experimental results of glycolytic oscillations with NADH fluorescence in DU145 prostate cancer cells (a), their frequency distribution (b), and the ratio of oscillatory
cells to total cells of N = 650 as function of time (c). The mean period was 46.2 ± 14.6 s, and
the maximum oscillatory ratio was 0.249. The cells were cultured at 37 °C, pre-incubated for 24 h
without glucose and with serum conditions (Glc−, FBS+) at 37 °C, and 20 mM glucose was added
for the experiment of glycolytic oscillations at 25 °C
T. Amemiya et al.
15.6 Glycolytic Oscillations in Prostate Cancer Cells
(DU145)
Prostate cancer, DU145 cells were also found to exhibit glycolytic oscillations for
the first time as shown in Fig. 15.3. Experimental methods for the oscillations in
DU145 cells were similar to those for HeLa cells [5]. We found that the periods of
oscillations (46.2 ± 14.6 s) were longer and the maximum of oscillatory ratio (0.249)
was smaller in DU145 cells than the periods (19.3 ± 6.30 s) and the oscillatory ratio
(0.355) in HeLa cells. DU145 cells also exhibited heterogeneities in the oscillations
in terms of the number of oscillatory cells, periods of oscillations, and duration of
oscillations, similar to HeLa cells [4, 5].
Interestingly, the Warburg effect, a common feature of cancer cells, is not observed
in prostate cancer cells [18]. In particular, early prostate cancers rely on lipids
and other energetic molecules, and not on aerobic respiration, for ATP production.
Fig. 15.3 Typical time series of experimental results of glycolytic oscillations with NADH fluorescence in DU145 prostate cancer cells (a), their frequency distribution (b), and the ratio of oscillatory
cells to total cells of N = 650 as function of time (c). The mean period was 46.2 ± 14.6 s, and
the maximum oscillatory ratio was 0.249. The cells were cultured at 37 °C, pre-incubated for 24 h
without glucose and with serum conditions (Glc−, FBS+) at 37 °C, and 20 mM glucose was added
for the experiment of glycolytic oscillations at 25 °C