13 Oscillations in Yeast Glycolysis
221
the Association-Induction (AI) hypothesis developed by Gilbert Ling [26, 29, 30,
32, 33], which treats the cellular interior in a resting cell as a highly structured
near-equilibrium system. According to the AI hypothesis intracellular water is in
a polarised state and most intracellular potassium ions are bound to cytoskeletal
proteins [32]. Interestingly the AI hypothesis, while largely ignored, has never been
refuted and we suggest that it may be relevant for all cellular types.
Acknowledgements The authors acknowledge the Danish Molecular Biomedical Imaging Centre
(DaMBIC, University of Southern Denmark) for the use of the bioimaging facilities. LFO was
supported by a grant from the Danish Council for Independent Research, Natural Sciences (DFF
4002-00465).
References
1. J. Aldridge, E. Pye, Cell density dependence of oscillatory metabolism. Nature 259(5545),
670–671 (1976)
2. A.Z. Andersen, A.K. Poulsen, J.C. Brasen, L.F. Olsen, On-line measurements of oscillating
mitochondrial membrane potential in glucose-fermenting Saccharomyces cerevisiae. Yeast
24(9), 731–739 (2007)
3. M. Aon et al., Dynamic regulation of yeast glycolytic oscillations by mitochondrial functions.
J. Cell Sci. 99(2), 325–334 (1991)
4. M. Aon, S. Cortassa, H. Westerhoff, K. van Dam, Synchrony and mutual stimulation of yeastcells during fast glycolytic oscillations. J. Gen. Microbiol. 138(10), 2219–2227 (1992)
5. L.A. Bagatolli, Laurdan fluorescence properties in membranes: a journey from the fluorometer
to the microscope, in Fluorescent Methods to Study Biological Membranes, ed. by Y. Mely, G.
Duportail (Springer, Berlin, Heidelberg, 2013), pp. 3–35
6. L.A. Bagatolli, R.P. Stock, The use of 6-acyl-2-(dimethylamino)naphthalenes as relaxation
probes of biological environments, in Perspectives on Fluorescence: A Tribute to Gregorio
Weber, Springer Series in Fluorescence, ed. by D.M. Jameson (Springer, Heidelberg, 2016),
pp. 197–216
7. S.F. Banani, H.O. Lee, A.A. Hyman, M.K. Rosen, Biomolecular condensates: organizers of
cellular biochemistry. Nature Rev. Mol. Cell Biol. 18(5), 285–298 (2017)
8. C. Bernard, Introduction a l’etude de la medicine experimentale (Macmillan, New York, 1927)
9. A. Betz, B. Chance, Influence of inhibitors and temperature on oscillation of reduced pyridine
nucleotides in yeast cells. Arch. Biochem. Biophys. 109(3), 579–584 (1965)
10. C.P. Brangwynne, T.J. Mitchison, A.A. Hyman, Active liquid-like behavior of nucleoli determines their size and shape in xenopus laevis oocytes. Proc. Natl. Acad. Sci. 108(11), 4334–4339
(2011)
11. B. Chance, R. Estabrook, A. Ghosh, Damped sinusoidal oscillations of cytoplasmic reduced
pyridine nucleotide in yeast cells. Proc. Natl. Acad. Sci. USA 51(6), 1244+ (1964)
12. B. Chance et al., Synchronization phenomena in oscillations of yeast cells and isolated mitochondria, in Biological and Biochemical Oscillators, ed. by B. Chance (Academic Press, New
York, 1973), pp. 285–300
13. F.A. Chandra, G. Buzi, J.C. Doyle, Glycolytic oscillations and limits on robust efficiency.
Science 333(6039), 187–192 (2011)
14. M. Chaplin, Do we underestimate the importance of water in cell biology? Nat. Rev. Mol. Cell
Biol. 7(11), 861–866 (2006)
15. N.A. Chebotareva, B.I. Kurganov, N.B. Livanova, Biochemical effects of molecular crowding.
Biochemistry (Moscow) 69(11), 1239–1251 (2004)
221
the Association-Induction (AI) hypothesis developed by Gilbert Ling [26, 29, 30,
32, 33], which treats the cellular interior in a resting cell as a highly structured
near-equilibrium system. According to the AI hypothesis intracellular water is in
a polarised state and most intracellular potassium ions are bound to cytoskeletal
proteins [32]. Interestingly the AI hypothesis, while largely ignored, has never been
refuted and we suggest that it may be relevant for all cellular types.
Acknowledgements The authors acknowledge the Danish Molecular Biomedical Imaging Centre
(DaMBIC, University of Southern Denmark) for the use of the bioimaging facilities. LFO was
supported by a grant from the Danish Council for Independent Research, Natural Sciences (DFF
4002-00465).
References
1. J. Aldridge, E. Pye, Cell density dependence of oscillatory metabolism. Nature 259(5545),
670–671 (1976)
2. A.Z. Andersen, A.K. Poulsen, J.C. Brasen, L.F. Olsen, On-line measurements of oscillating
mitochondrial membrane potential in glucose-fermenting Saccharomyces cerevisiae. Yeast
24(9), 731–739 (2007)
3. M. Aon et al., Dynamic regulation of yeast glycolytic oscillations by mitochondrial functions.
J. Cell Sci. 99(2), 325–334 (1991)
4. M. Aon, S. Cortassa, H. Westerhoff, K. van Dam, Synchrony and mutual stimulation of yeastcells during fast glycolytic oscillations. J. Gen. Microbiol. 138(10), 2219–2227 (1992)
5. L.A. Bagatolli, Laurdan fluorescence properties in membranes: a journey from the fluorometer
to the microscope, in Fluorescent Methods to Study Biological Membranes, ed. by Y. Mely, G.
Duportail (Springer, Berlin, Heidelberg, 2013), pp. 3–35
6. L.A. Bagatolli, R.P. Stock, The use of 6-acyl-2-(dimethylamino)naphthalenes as relaxation
probes of biological environments, in Perspectives on Fluorescence: A Tribute to Gregorio
Weber, Springer Series in Fluorescence, ed. by D.M. Jameson (Springer, Heidelberg, 2016),
pp. 197–216
7. S.F. Banani, H.O. Lee, A.A. Hyman, M.K. Rosen, Biomolecular condensates: organizers of
cellular biochemistry. Nature Rev. Mol. Cell Biol. 18(5), 285–298 (2017)
8. C. Bernard, Introduction a l’etude de la medicine experimentale (Macmillan, New York, 1927)
9. A. Betz, B. Chance, Influence of inhibitors and temperature on oscillation of reduced pyridine
nucleotides in yeast cells. Arch. Biochem. Biophys. 109(3), 579–584 (1965)
10. C.P. Brangwynne, T.J. Mitchison, A.A. Hyman, Active liquid-like behavior of nucleoli determines their size and shape in xenopus laevis oocytes. Proc. Natl. Acad. Sci. 108(11), 4334–4339
(2011)
11. B. Chance, R. Estabrook, A. Ghosh, Damped sinusoidal oscillations of cytoplasmic reduced
pyridine nucleotide in yeast cells. Proc. Natl. Acad. Sci. USA 51(6), 1244+ (1964)
12. B. Chance et al., Synchronization phenomena in oscillations of yeast cells and isolated mitochondria, in Biological and Biochemical Oscillators, ed. by B. Chance (Academic Press, New
York, 1973), pp. 285–300
13. F.A. Chandra, G. Buzi, J.C. Doyle, Glycolytic oscillations and limits on robust efficiency.
Science 333(6039), 187–192 (2011)
14. M. Chaplin, Do we underestimate the importance of water in cell biology? Nat. Rev. Mol. Cell
Biol. 7(11), 861–866 (2006)
15. N.A. Chebotareva, B.I. Kurganov, N.B. Livanova, Biochemical effects of molecular crowding.
Biochemistry (Moscow) 69(11), 1239–1251 (2004)