Atomic Force Microscopy-Based Force
Spectroscopy and Its Various
Applications
Jyoti Jaisawal, Amit Kumar Srivastav, Dheeraj K. Singh, and Umesh Kumar
Abstract The complex interaction between various biomolecules and material interfaces at molecular level requires the deeper understanding of different sciences like
materials science, nanotechnology, biophysics, biomedicine, and analytical science.
Microscopy is very common techniques among these different scientific fields where
studies depend upon resolution of Image obtained. None of these microscopy techniques can give both imaging and spectroscopic information with resolution down
to nm. Atomic force microscopy is among few scientific tools that can record both
imaging and spectroscopic data with high resolution. One such sensitive tool is
Atomic force microscopy-based force spectroscopy (AFM-FS), which can be used
to probe the intermolecular forces between the biomolecules and interfaces of the
various materials ranging from the hard-inorganic surfaces to various soft biologicalsubstrates. The present chapter emphasis the utilization of AFM-FS to understand
the biomolecules and materials interfaces interaction at the single-molecule level
with emphasis on the basic principles and application of this methodology and some
of the practical aspects involved. We believe that the present chapter will be beneficent to the researchers in this field about the fundamental molecular interactions
and utilization of AFM-FS to develop the in-depth understanding of the biological
functions of various bio-hybrid materials.
Keywords AFM · AFM-FS · Material science · Nanotechnology
1 Introduction
Atomic force microscope (AFM) is a non-conventional microscope which generates
images by probing forces operating at the atomic level. These images can be obtained
J. Jaisawal · A. K. Srivastav · U. Kumar (B)
School of Nano Sciences, Central University of Gujarat, Gandhinagar, Gujarat 382030, India
e-mail: umesh.kumar@cug.ac.in
D. K. Singh
Department of Physics, Institute of Infrastructure Technology Research and Management
(IITRAM), Ahmedabad, Gujarat 380026, India
© The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2021
D. K. Singh et al. (eds.), Modern Techniques of Spectroscopy, Progress in Optical Science
and Photonics 13, https://doi.org/10.1007/978-981-33-6084-6_21
585
Spectroscopy and Its Various
Applications
Jyoti Jaisawal, Amit Kumar Srivastav, Dheeraj K. Singh, and Umesh Kumar
Abstract The complex interaction between various biomolecules and material interfaces at molecular level requires the deeper understanding of different sciences like
materials science, nanotechnology, biophysics, biomedicine, and analytical science.
Microscopy is very common techniques among these different scientific fields where
studies depend upon resolution of Image obtained. None of these microscopy techniques can give both imaging and spectroscopic information with resolution down
to nm. Atomic force microscopy is among few scientific tools that can record both
imaging and spectroscopic data with high resolution. One such sensitive tool is
Atomic force microscopy-based force spectroscopy (AFM-FS), which can be used
to probe the intermolecular forces between the biomolecules and interfaces of the
various materials ranging from the hard-inorganic surfaces to various soft biologicalsubstrates. The present chapter emphasis the utilization of AFM-FS to understand
the biomolecules and materials interfaces interaction at the single-molecule level
with emphasis on the basic principles and application of this methodology and some
of the practical aspects involved. We believe that the present chapter will be beneficent to the researchers in this field about the fundamental molecular interactions
and utilization of AFM-FS to develop the in-depth understanding of the biological
functions of various bio-hybrid materials.
Keywords AFM · AFM-FS · Material science · Nanotechnology
1 Introduction
Atomic force microscope (AFM) is a non-conventional microscope which generates
images by probing forces operating at the atomic level. These images can be obtained
J. Jaisawal · A. K. Srivastav · U. Kumar (B)
School of Nano Sciences, Central University of Gujarat, Gandhinagar, Gujarat 382030, India
e-mail: umesh.kumar@cug.ac.in
D. K. Singh
Department of Physics, Institute of Infrastructure Technology Research and Management
(IITRAM), Ahmedabad, Gujarat 380026, India
© The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2021
D. K. Singh et al. (eds.), Modern Techniques of Spectroscopy, Progress in Optical Science
and Photonics 13, https://doi.org/10.1007/978-981-33-6084-6_21
585