freshwater aquatic eco-toxicity (FEP), marine aquatic eco-toxicity (MEP) and terrestrial eco-toxicity (TEP). SimaPro 8.0.2 was used for the implementation [18].
2.2 Social Assessment
The social dimension is frequently considered as the weakest pillar of sustainable
development, as reflected by the limited analytical and theoretical tools for its
evaluation [19]. In order to develop a standardized methodology, UNEP-SETAC
published the Guidelines for Social Life Cycle Assessment of Products, together
with the methodological sheets for subcategories in Social Life Cycle Assessment
(SLCA) [8, 20]. The methodological sheets contain all the necessary information to
collect data for 31 defined impact sub-categories, which correspond to relevant
characterised social issues. The sub-categories representing social impacts are
classified into five stakeholder categories: workers, consumers, local community,
society and value chain actors. The given information includes a definition of each
sub-category and an explanation of issues associated with it, as well as examples of
inventory indicators, units of measurement and data sources [20].
ASTAXANTHIN IN
OLEORESIN (10%)
AIR, SOIL & WATER
EMISSIONS
FOREGROUND SYSTEM
BACKGROUND
SYSTEM
RAW MATERIALS, WATER & FOSSIL FUELS
CHEMICALS
(Nutrients &
Solvents)
MACHINERY
ELECTRICITY
AIR SUPPLY
WASTES TO
TREATMENT
BYPRODUCT
(FERTILIZER)
WATER SUPPLY
SUBSYSTEM 1: CLEAN.
AND STERILIZ.
SUBSYSTEM 2: PREPARATION
OF CULTURE MEDIUM
SUBSYSTEM 3:
CULTIVATION
SUBSYSTEM 4:
HARVESTING
SUBSYSTEM 5: EXTRACTION
REACTOR
STERILIZATION
GROWTH IN PBR
CENTRIFUGATION
SPRAY DRYING/
FREEZE-DRYING
ADDITION OF
NUTRIENTS
TO WATER
AUTOCLAVING
INOCULUM
ADDITION
(CO-)SOLVENT ADDITION
VORTEX MIXING
CENTRIFUGATION
T
T Transport
T
INCUBATION
REVERSE OSMOSIS FILTER
UV FILTER
SETTLING
STRESS STAGE
SETTLING
SUPERCRITICAL CO 2
EXTRACTION
Lab-scale
Fig. 1 Process chain and system boundaries for the production of H. pluvialis astaxanthin (blocks
in dark grey correspond to steps that are specific to the pilot-scale production, blocks with dotted
lines correspond to steps that are specific of lab- and semi-pilot production)
478
P. Pérez-López et al.
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