DETERMINATION OF PEPTIDE SEQUENCE
547
The N -terminal amino acid can be ascertained by
the Sanger method. Enzymic cleavage using either
chymotrypsin or trypsin will break the peptide into
smaller fragments. The fragments obtained will be
different, depending on the enzyme and its specificity.
The smaller fragments are then each sequenced by the
Edman technique. C-Terminal residues in the smaller
peptides can be related to knowledge of the enzyme
cleavage sites; this may point to the C-terminal
residue of the full peptide if it does not correspond to
an enzymic cleavage site. The full sequence can be
deduced from these fragments by lining up matching
sequences of overlapping portions.
547
The N -terminal amino acid can be ascertained by
the Sanger method. Enzymic cleavage using either
chymotrypsin or trypsin will break the peptide into
smaller fragments. The fragments obtained will be
different, depending on the enzyme and its specificity.
The smaller fragments are then each sequenced by the
Edman technique. C-Terminal residues in the smaller
peptides can be related to knowledge of the enzyme
cleavage sites; this may point to the C-terminal
residue of the full peptide if it does not correspond to
an enzymic cleavage site. The full sequence can be
deduced from these fragments by lining up matching
sequences of overlapping portions.