76
C. Elizabeth Rani et al.
8.4.3 Hydrocarbon Overlay Agar Method
Hydrocarbon overlay agar method is a quantitative method for the detection of hydrocarbon emulsifiers. It shows the hydrocarbon degrading capability of on organism.
Clear zones were observed and the test organism showed 0.8 mm clearance zone of
diameter.
8.4.4 Emulsification Index (E24)
For this assay, crude oil was used as hydrophobic substrate to study the emulsification activity of the crude sample. Emulsification index >30% considered as high
bisurfactant activity was reported by (Nayarisseri et al. 2018). The results of the
extracted crude sample showing 66.6% for the biosurfactant activity (Fig. 3).
Fig. 3 Emulsification test
for the screening of
biosurfactant
C. Elizabeth Rani et al.
8.4.3 Hydrocarbon Overlay Agar Method
Hydrocarbon overlay agar method is a quantitative method for the detection of hydrocarbon emulsifiers. It shows the hydrocarbon degrading capability of on organism.
Clear zones were observed and the test organism showed 0.8 mm clearance zone of
diameter.
8.4.4 Emulsification Index (E24)
For this assay, crude oil was used as hydrophobic substrate to study the emulsification activity of the crude sample. Emulsification index >30% considered as high
bisurfactant activity was reported by (Nayarisseri et al. 2018). The results of the
extracted crude sample showing 66.6% for the biosurfactant activity (Fig. 3).
Fig. 3 Emulsification test
for the screening of
biosurfactant