Bioremediation of Heavy Metals and Toxic Chemicals …
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and biomass was studied. Previously used untreated, treated with biosurfactant and
treated with biomass samples were taken to FTIR analysis. No changes were made in
the previously treated strategy and the same samples were taken for FTIR to determine
the presence of functional groups in untreated and the absence of functional groups
in the treated samples with biosurfactant and biomass respectively.
7 Biosurfactant as Stain Decolorizer Against Azo Dye (Red)
To check the ability of the biosurfactant as a vital ingredient in detergent, it was
studied by artificially stained the white cloth pieces with commercially available Azo
dye (red). For this study, initially 0.05% of 3 drops of synthetic azo dye was used to
stain on white cloth pieces to reveal the biosurfactant potential on stain removal or
decolorizer. This was done by adding Equal volume of produced biosurfactant in the
stained cloth and control was maintained. The stained cloths were kept undisturbed
hardly for about 30 min and changes were observed visibly.
8 Results
8.1 Isolation of Indigenous Bacteria
Pour plate technique of the collected water sample gave various colonies with distinct
colony morphologies. The morphology was studied and based on its appearance the
selected organism was streaked on nutrient agar plates and allowed to incubate for
overnight at 37
◦ C in and hence pure culture of selected strain was achieved.
8.2 Identification of Isolated Bacteria
Phenotypic characterization:
The Gram stained cells were observed under the light microscope at 100 × using
oil immersion. The colour and shape of the cells were studied. Gram negative rod
shaped cells were observed as pink colour (Table 1). Other phenotypic methods as
per Bergy’s manuel was carried out and the results were tabulated.
From the above observation and results the unknown organism was identified as
Pseudomonas aeruginosa.
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and biomass was studied. Previously used untreated, treated with biosurfactant and
treated with biomass samples were taken to FTIR analysis. No changes were made in
the previously treated strategy and the same samples were taken for FTIR to determine
the presence of functional groups in untreated and the absence of functional groups
in the treated samples with biosurfactant and biomass respectively.
7 Biosurfactant as Stain Decolorizer Against Azo Dye (Red)
To check the ability of the biosurfactant as a vital ingredient in detergent, it was
studied by artificially stained the white cloth pieces with commercially available Azo
dye (red). For this study, initially 0.05% of 3 drops of synthetic azo dye was used to
stain on white cloth pieces to reveal the biosurfactant potential on stain removal or
decolorizer. This was done by adding Equal volume of produced biosurfactant in the
stained cloth and control was maintained. The stained cloths were kept undisturbed
hardly for about 30 min and changes were observed visibly.
8 Results
8.1 Isolation of Indigenous Bacteria
Pour plate technique of the collected water sample gave various colonies with distinct
colony morphologies. The morphology was studied and based on its appearance the
selected organism was streaked on nutrient agar plates and allowed to incubate for
overnight at 37
◦ C in and hence pure culture of selected strain was achieved.
8.2 Identification of Isolated Bacteria
Phenotypic characterization:
The Gram stained cells were observed under the light microscope at 100 × using
oil immersion. The colour and shape of the cells were studied. Gram negative rod
shaped cells were observed as pink colour (Table 1). Other phenotypic methods as
per Bergy’s manuel was carried out and the results were tabulated.
From the above observation and results the unknown organism was identified as
Pseudomonas aeruginosa.